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how to filter results from structure search using FASTA input and custom annotation db

Open jflucier opened this issue 6 months ago • 0 comments

Hi,

I have looked at answer provided in issue #167

I understand that the main criteria for filtering is evalue based on the response provided in issue mentionned above. When I look at my results, I see some interesting results but evalue is not so good:

query SLCT-1 target BIHOEHLG_02031 fident 0.078 alnlen 610 mismatch 526 gapopen 0 qstart 24 qend 633 tstart 1 tend 572 evalue 0.1708 bits 103 qcov 0.825 tcov 0.888

So the query and target have a nice coverage but evalue turns out not so good. This next example is the hit with the best bit score:

query SLCT-1 target BIHOEHLG_00538 fident 0.314 alnlen 371 mismatch 217 gapopen 0 qstart 366 qend 736 tstart 32 tend 349 evalue 1.35E-28 bits 1214 qcov 0.502 tcov 0.909

The query coverrage is only at 50%.

If you can provide me with any information on how to interperet correctly results this would help me for the filtering step.

Thank you in advance for your help, JF

jflucier avatar Aug 01 '24 17:08 jflucier