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extract question: How to extract the unclassified reads

Open grendon opened this issue 3 months ago • 1 comments

Hi,

The metabuli report has taxid=0 for the unclassified category.
But when I try that value in the extract command to get the unclassified reads, it doesn't work. Is there a way to extract the unclassified reads?

My version of metabuli is 1.1.0


metabuli extract $r1 $r2 $classout $DBDIR --tax-id 0 --seq-mode 2 --extract-format 2


Metabuli Version (commit):                        	1.1.0
Directory where the taxonomy dump files are stored	
Sequencing type                                   	2
Tax. ID of clade to be extracted                  	0
0: original format, 1: FASTA, 2: FASTQ            	2

Please provide a target taxon ID with --tax-id parameter.

grendon avatar Oct 01 '25 20:10 grendon

Now you can put --tax-id -1 to extract unclassified reads. please download and test from https://dev.mmseqs.com/metabuli/

jaebeom-kim avatar Oct 27 '25 01:10 jaebeom-kim