tutorial dataset
Hallo, i was running popscle demuxlet on the tutorial dataset, which detected 6 doublets. However, based on the .best file in the goole drive, the dataset has roughly 40 doublets. Why was a difference between 2 result? Does dsc-pileup change the result eventually?
Thanks and best wishes Xichen
Most likely, it is because the default parameter was changed. The default parameters are not identical between versions
On Fri, Sep 9, 2022, 7:43 PM Xichen Wu @.***> wrote:
Hallo, i was running popscle demuxlet on the tutorial dataset, which detected 6 doublets. However, based on the .best file in the goole drive, the dataset has roughly 40 doublets. Why was a difference between 2 result? Does dsc-pipeup change the result eventuallu?
Thanks and best wishes Xichen
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Thank you so much for the quick response! This would be helpful! I was struggling to have the same result.
Sorry but another question, can I ask which parameter has changed? I am thinking maybe the alpha value in this case.