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inconsistency using fragment or whole protein

Open twaksman001 opened this issue 1 year ago • 0 comments

I used HHBlits via the MPI website

If I submit whole protein sequence - 10000 matches, mostly kinase proteins, and the alignment is to a subsection of the query.

If I resubmit this subsection, <50 matches and no kinases.

Please can you explain how this happens?

Protein sequence: NPLCPYMNRGNPNLLPQQKSSTTQGDQVYAPSMPQFSMEGNRQMYPMPPPMMPPPPMMQGFQPPFPFNPMNQQQLSMLLMSSYARAMALAQLMNMQNRFQFYQPNQYSDPAINNYSSMPATLPQQPQVDLQNNLKTEMSPEQEKQGEPGEPRESELETSQQQGELQQQEEPLNSGEGQETEVSGEREEPETRDQDILEKRKMQRALDIEIERRERLQKRKAEENELERQQKEMERMLREEEEMKRQEEETKRQILEEERKEREEKEKDIKLRQEEELRFKKIEAEKKKRNQVNNKPQGTSVRRQRKQKHTKKTVTEDIIERL

twaksman001 avatar Feb 27 '23 09:02 twaksman001