build customized databases

[Wanli-HE]

:exclamation: Make to check out our User Guide.

hi ! i am trying to build a customized databases, using the workflow that recorded in "user guide"

first step using "ffindex_from_fasta" to convert the input FASTA file. command like below: "ffindex_from_fasta -s all-neighbours-refer-and-metagenome.fa.ffdata all-neighbours-refer-and-metagenome.fa.ffindex all-neighbours-refer-and-metagenome.fa"

it is works. but in the next step, using "hhblits_omp" to get MSA file. "hhblits_omp -i all-neighbours-refer-and-metagenome.fa -d /home/projects/ku_00041/data/hh-suite-database/UniRef30_2020_06 -oa3m all-neighbours-refer-and-metagenome_a3m_ww_ss -n 2 -cpu 35"

but it not working, the error like: ------------------------------------------------------------------error--------------------------------------------------------------

• 09:13:31.134 INFO: EIC29417.1--(EIC29420.1) is in A2M, A3M or FASTA format

• 09:13:31.162 INFO: Iteration 1

• 09:13:31.697 ERROR: Error in /opt/conda/conda-bld/hhsuite_1616660820288/work/src/hhfunc.cpp:83: ReadQueryFile:

' 09:13:31.697 ERROR: unrecognized input file format in '3300029656.a:Ga0245165_100003_39¸

• 09:13:31.697 ERROR: line = 4--(DD08414.1)

• 09:13:31.698 ERROR: Error in /opt/conda/conda-bld/hhsuite_1616660820288/work/src/hhfunc.cpp:83: ReadQueryFile:

• 09:13:31.698 ERROR: unrecognized input file format in '3300011605.a:Ga0120982_100063_27*'

• 09:13:31.698 ERROR: line = --(CF53703.1)

• 09:13:31.698 INFO: CRP04010.1--(CRP04742.1) is in A2M, A3M or FASTA format

• 09:13:31.698 ERROR: Error in /opt/conda/conda-bld/hhsuite_1616660820288/work/src/hhfunc.cpp:83: ReadQueryFile:

• 09:13:31.698 ERROR: unrecognized input file format in 'AEY01231.1--(AEY01220.1)'

• 09:13:31.698 ERROR: line = -AEY01231.1--(AEY01220.1)

---

here is my fasta file:

and the version is 3.30

so do you have any idea about this error.

best, wanli

[Wanli-HE]

ok i had figure out, it is due to the length of protein name