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Published 20 hours ago • verified publisher icon soedinglab

HHblits process killed

Open sgorman90 opened this issue 3 years ago • 5 comments

Hi All,

I'm using HHblits in the context of Porter5 to help predict secondary structure. Porter executes HHblits with the flags -cpu 2 -n 3 -maxfilt 150000 -maxmem 8 -v 2. My system runs Pop!_OS linux with 16 gb memory and a Ryzen 5 5600x 6 core processor.

After I run a particular amino acid sequence (that I can make available if needed) HHblits ends early and the log file says the process was "Killed" (see below). Any advice would be greatly appreciated!

  • 13:39:28.388 INFO: Search results will be written to test011_TCF3_ZNF384.hhr

  • 13:39:29.524 INFO: Searching 8290206 column state sequences.

  • 13:39:29.552 INFO: test011_TCF3_ZNF384.fasta is in A2M, A3M or FASTA format

  • 13:39:29.553 INFO: Iteration 1

  • 13:39:29.853 INFO: Prefiltering database

  • 13:41:21.032 INFO: HMMs passed 1st prefilter (gapless profile-profile alignment) : 556889

  • 13:41:28.048 WARNING: database contains sequences that exceeds maximum allowed size (maxres = 20001). Maxres can be increased with parameter -maxres.

  • 13:41:28.153 INFO: HMMs passed 2nd prefilter (gapped profile-profile alignment) : 99165

  • 13:41:28.153 INFO: HMMs passed 2nd prefilter and not found in previous iterations : 99165

  • 13:41:28.153 INFO: Scoring 99165 HMMs using HMM-HMM Viterbi alignment

  • 13:41:28.180 INFO: Alternative alignment: 0

  • 13:41:28.512 INFO: 2000 alignments done

  • 13:41:28.958 INFO: 4000 alignments done

  • 13:41:29.513 INFO: 6000 alignments done

  • 13:41:30.172 INFO: 8000 alignments done

  • 13:41:30.940 INFO: 10000 alignments done

  • 13:41:31.826 INFO: 12000 alignments done

  • 13:41:32.833 INFO: 14000 alignments done

  • 13:41:33.956 INFO: 16000 alignments done

  • 13:41:35.187 INFO: 18000 alignments done

  • 13:41:36.559 INFO: 20000 alignments done

  • 13:41:38.078 INFO: 22000 alignments done

  • 13:41:39.746 INFO: 24000 alignments done

  • 13:41:41.591 INFO: 26000 alignments done

  • 13:41:43.606 INFO: 28000 alignments done

  • 13:41:45.820 INFO: 30000 alignments done

  • 13:41:48.245 INFO: 32000 alignments done

  • 13:41:50.946 INFO: 34000 alignments done

  • 13:41:53.940 INFO: 36000 alignments done

  • 13:41:57.304 INFO: 38000 alignments done

  • 13:42:01.173 INFO: 40000 alignments done

  • 13:42:05.471 INFO: 42000 alignments done

  • 13:42:09.795 INFO: 44000 alignments done

  • 13:42:12.701 INFO: 46000 alignments done

  • 13:42:14.711 INFO: 48000 alignments done

  • 13:42:16.676 INFO: 50000 alignments done

  • 13:42:18.732 INFO: 52000 alignments done

  • 13:42:20.696 INFO: 54000 alignments done

  • 13:42:20.699 WARNING: Number of match columns too large. Only first 19999 match columns will be kept!

  • 13:42:20.704 WARNING: Number of match columns too large. Only first 19999 match columns will be kept!

  • 13:42:22.779 INFO: 56000 alignments done

  • 13:42:22.783 WARNING: Number of match columns too large. Only first 19999 match columns will be kept!

  • 13:42:24.820 INFO: 58000 alignments done

  • 13:42:26.784 INFO: 60000 alignments done

  • 13:42:28.723 INFO: 62000 alignments done

  • 13:42:30.772 INFO: 64000 alignments done

  • 13:42:32.774 INFO: 66000 alignments done

  • 13:42:34.843 INFO: 68000 alignments done

  • 13:42:36.799 INFO: 70000 alignments done

  • 13:42:38.719 INFO: 72000 alignments done

  • 13:42:40.700 INFO: 74000 alignments done

  • 13:42:42.624 INFO: 76000 alignments done

  • 13:42:44.604 INFO: 78000 alignments done

  • 13:42:46.505 INFO: 80000 alignments done

  • 13:42:48.453 INFO: 82000 alignments done

  • 13:42:50.438 INFO: 84000 alignments done

  • 13:42:52.390 INFO: 86000 alignments done

  • 13:42:54.348 INFO: 88000 alignments done

  • 13:42:56.306 INFO: 90000 alignments done

  • 13:42:58.295 INFO: 92000 alignments done

  • 13:42:58.297 INFO: Stop after DB-HHM: 92000 because early stop 18.7068 < filter cutoff 20

  • 13:42:58.300 INFO: Alternative alignment: 1

  • 13:42:58.303 WARNING: Number of match columns too large. Only first 19999 match columns will be kept!

  • 13:42:58.308 WARNING: Number of match columns too large. Only first 19999 match columns will be kept!

  • 13:42:58.313 WARNING: Number of match columns too large. Only first 19999 match columns will be kept!

  • 13:44:28.849 INFO: 87853 alignments done

  • 13:44:28.985 INFO: Alternative alignment: 2

  • 13:44:28.988 WARNING: Number of match columns too large. Only first 19999 match columns will be kept!

  • 13:44:28.993 WARNING: Number of match columns too large. Only first 19999 match columns will be kept!

  • 13:44:28.000 WARNING: Number of match columns too large. Only first 19999 match columns will be kept!

  • 13:45:41.853 INFO: 61705 alignments done

  • 13:45:41.914 INFO: Alternative alignment: 3

  • 13:45:41.917 WARNING: Number of match columns too large. Only first 19999 match columns will be kept!

  • 13:45:41.921 WARNING: Number of match columns too large. Only first 19999 match columns will be kept!

  • 13:45:41.927 WARNING: Number of match columns too large. Only first 19999 match columns will be kept!

  • 13:46:41.174 INFO: 47257 alignments done

Killed

sgorman90 avatar Mar 28 '21 19:03 sgorman90

16GB RAM are probably too little for a database the size of the Uniprot20. The best solution would be to use a different machine or install more RAM.

You could try to restrict maximum alignment length by lowering -maxres, that might result in some potentially good hits not being found anymore though.

milot-mirdita avatar Mar 28 '21 23:03 milot-mirdita

I'm curious what the rule of thumb might be in deciding how much ram to use. Would something like 32 gb work better?

Thanks for your response!

Scott D. Gorman, Ph.D.

Postdoctoral Research Associate | Co-Department Rep | Structural Biology Department

St. Jude Children’s Research Hospital

o: 901-595-6136 | c: 617-842-0947 | Kriwacki Labhttp://www.stjuderesearch.org/kriwacki

From: Milot Mirdita @.> Sent: Sunday, March 28, 2021 6:44 PM To: soedinglab/hh-suite @.> Cc: Gorman, Scott [email protected]; Author @.***> Subject: Re: [soedinglab/hh-suite] HHblits process killed (#253)

Caution: External Sender. Do not open unless you know the content is safe.

16GB RAM are probably too little for a database the size of the Uniprot20. The best solution would be to use a different machine or install more RAM.

You could try to restrict maximum alignment length by lowering -maxres, that might result in some potentially good hits not being found anymore though.

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sgorman90 avatar Mar 29 '21 00:03 sgorman90

You could try using the SSE2 binaries, they need a bit less memory at the cost of a bit speed. I guess that you are quite close to the brink right now. I would go straight for 64GB though as RAM is relatively cheap these days.

milot-mirdita avatar Mar 29 '21 13:03 milot-mirdita

I'm using a machine with 504 Gb of RAM and getting the same killed signal. The vmem used, based on htop is ~214 Gb. I'm querying against UniRef30. If I use a query of 336 AA, then hhblits completes successfully, but if I use a query that is 796 AA, then I get the killed signal.

Just before I get the killed signal, resident memory does quickly climb up to ~20 Gb (according to htop), and I get a lot of warnings:

- 07:36:42.879 INFO: HMMs passed 1st prefilter (gapless profile-profile alignment)  : 9214776

- 07:37:31.854 WARNING: Number of hits passing 2nd prefilter (reduced from 710225 to allowed maximum of 20000).
You can increase the allowed maximum using the -maxfilt <max> option.

- 07:37:31.921 WARNING: database contains sequences that exceed maximum allowed size (maxres = 20001). Max sequence length can be increased with parameter -maxres.

- 07:37:31.921 INFO: HMMs passed 2nd prefilter (gapped profile-profile alignment)   : 20000

- 07:37:31.921 INFO: HMMs passed 2nd prefilter and not found in previous iterations : 19795

- 07:37:31.921 INFO: Scoring 19795 HMMs using HMM-HMM Viterbi alignment

- 07:37:32.607 INFO: Alternative alignment: 0

- 07:37:32.658 WARNING: Number of match columns too large. Only first 19999 match columns will be kept!

- 07:37:32.692 WARNING: Number of match columns too large. Only first 19999 match columns will be kept!

- 07:37:34.370 INFO: 2000 alignments done

- 07:37:34.429 WARNING: Number of match columns too large. Only first 19999 match columns will be kept!

- 07:37:36.024 INFO: 4000 alignments done

- 07:37:36.030 WARNING: maximum number of residues 32763 exceeded in sequence UniRef100_A0A6J3RMW2_consensus

- 07:37:36.030 WARNING: maximum number of residues 32763 exceeded in sequence UniRef100_A0A6J3RMW2 LOW QUALITY PROTEIN: titin n=1 Tax=Tursiops truncatus TaxID=9739 RepID=A0A6J3RMW2_TURTR

- 07:37:36.030 WARNING: maximum number of residues 32763 exceeded in sequence UniRef100_UPI000F443DA9 titin n=1 Tax=Lagenorhynchus obliquidens TaxID=90247 RepID=UPI000F443DA9

- 07:37:36.030 WARNING: maximum number of residues 32763 exceeded in sequence UniRef100_UPI00122FA9B9 LOW QUALITY PROTEIN: titin n=1 Tax=Globicephala melas TaxID=9731 RepID=UPI00122FA9B9

- 07:37:36.030 WARNING: maximum number of residues 32763 exceeded in sequence UniRef100_UPI0014411C62 titin n=1 Tax=Orcinus orca TaxID=9733 RepID=UPI0014411C62

- 07:37:36.034 WARNING: Number of match columns too large. Only first 19999 match columns will be kept!

- 07:37:36.063 WARNING: Number of match columns too large. Only first 19999 match columns will be kept!

- 07:37:36.090 WARNING: Number of match columns too large. Only first 19999 match columns will be kept!

- 07:37:38.162 INFO: 6000 alignments done

- 07:37:38.198 WARNING: Number of match columns too large. Only first 19999 match columns will be kept!

- 07:37:38.256 WARNING: Number of match columns too large. Only first 19999 match columns will be kept!

- 07:37:40.592 INFO: 8000 alignments done

- 07:37:40.654 WARNING: Number of match columns too large. Only first 19999 match columns will be kept!

- 07:37:42.708 INFO: 10000 alignments done

- 07:37:42.749 WARNING: maximum number of residues 32763 exceeded in sequence UniRef100_A0A5E4B6R9_consensus

- 07:37:42.749 WARNING: maximum number of residues 32763 exceeded in sequence UniRef100_A0A5E4B6R9 Uncharacterized protein n=2 Tax=Marmota monax TaxID=9995 RepID=A0A5E4B6R9_MARMO

- 07:37:42.749 WARNING: maximum number of residues 32763 exceeded in sequence UniRef100_A0A5E4B6S7 Uncharacterized protein n=1 Tax=Marmota monax TaxID=9995 RepID=A0A5E4B6S7_MARMO

- 07:37:42.749 WARNING: maximum number of residues 32763 exceeded in sequence UniRef100_A0A5E4B9M5 Uncharacterized protein n=1 Tax=Marmota monax TaxID=9995 RepID=A0A5E4B9M5_MARMO

- 07:37:42.752 WARNING: Number of match columns too large. Only first 19999 match columns will be kept!

- 07:37:42.778 WARNING: maximum number of residues 32763 exceeded in sequence UniRef100_UPI000B511B8A_consensus

- 07:37:42.778 WARNING: maximum number of residues 32763 exceeded in sequence UniRef100_UPI000B511B8A titin isoform X1 n=1 Tax=Aotus nancymaae TaxID=37293 RepID=UPI000B511B8A

- 07:37:42.779 WARNING: maximum number of residues 32763 exceeded in sequence UniRef100_UPI00193DD89E LOW QUALITY PROTEIN: titin n=1 Tax=Saimiri boliviensis boliviensis TaxID=39432 RepID=UPI00193DD89E

- 07:37:42.781 WARNING: Number of match columns too large. Only first 19999 match columns will be kept!

- 07:37:44.765 INFO: 12000 alignments done

- 07:37:44.776 WARNING: Number of match columns too large. Only first 19999 match columns will be kept!

- 07:37:44.804 WARNING: Number of match columns too large. Only first 19999 match columns will be kept!

- 07:37:46.787 INFO: 14000 alignments done

- 07:37:46.796 WARNING: maximum number of residues 32763 exceeded in sequence UniRef100_A0A480SN35_consensus

- 07:37:46.797 WARNING: maximum number of residues 32763 exceeded in sequence UniRef100_A0A480SN35 Titin isoform X6 n=1 Tax=Sus scrofa TaxID=9823 RepID=A0A480SN35_PIG

- 07:37:46.797 WARNING: maximum number of residues 32763 exceeded in sequence UniRef100_UPI000A2B0F7C LOW QUALITY PROTEIN: titin n=1 Tax=Sus scrofa TaxID=9823 RepID=UPI000A2B0F7C

- 07:37:46.797 WARNING: maximum number of residues 32763 exceeded in sequence UniRef100_UPI0014550278 LOW QUALITY PROTEIN: titin n=1 Tax=Fukomys damarensis TaxID=885580 RepID=UPI0014550278

- 07:37:46.800 WARNING: Number of match columns too large. Only first 19999 match columns will be kept!

- 07:37:49.060 INFO: 16000 alignments done

- 07:37:49.119 WARNING: Number of match columns too large. Only first 19999 match columns will be kept!

- 07:37:49.165 WARNING: Number of match columns too large. Only first 19999 match columns will be kept!

- 07:37:51.143 INFO: 18000 alignments done

- 07:37:51.268 WARNING: Number of match columns too large. Only first 19999 match columns will be kept!

- 07:37:53.422 INFO: 19795 alignments done

- 07:37:53.430 INFO: Alternative alignment: 1

- 07:37:53.435 WARNING: Number of match columns too large. Only first 19999 match columns will be kept!

- 07:37:53.474 WARNING: Number of match columns too large. Only first 19999 match columns will be kept!

- 07:37:57.086 INFO: 5763 alignments done

- 07:37:57.114 INFO: Alternative alignment: 2

Killed

nick-youngblut avatar Dec 11 '21 06:12 nick-youngblut

@nick-youngblut 遇到了和您类似的问题 13:29:14.096 WARNING: Warning in /home/vsts/work/1/s/src/hhalignment.cpp:3525: MergeMasterSlave:

  • 13:29:14.096 WARNING: maximum number of 65535 sequences exceeded while reading tr|T2MAJ6|T2MAJ6_HYDVU. Skipping all following sequences of this MSA

  • 13:29:14.096 WARNING: Warning in /home/vsts/work/1/s/src/hhalignment.cpp:3525: MergeMasterSlave:

  • 13:29:14.096 WARNING: maximum number of 65535 sequences exceeded while reading tr|A0A2L2YWY1|A0A2L2YWY1_PARTP. Skipping all following sequences of this MSA

  • 13:29:14.096 WARNING: Warning in /home/vsts/work/1/s/src/hhalignment.cpp:3525: MergeMasterSlave:

  • 13:29:14.096 WARNING: maximum number of 65535 sequences exceeded while reading tr|A0A0B2R0Y4|A0A0B2R0Y4_GLYSO. Skipping all following sequences of this MSA

  • 13:29:14.096 WARNING: Warning in /home/vsts/work/1/s/src/hhalignment.cpp:3525: MergeMasterSlave:

  • 13:29:14.096 WARNING: maximum number of 65535 sequences exceeded while reading tr|A0A2P4MEM7|A0A2P4MEM7_QUESU. Skipping all following sequences of this MSA

  • 13:29:14.096 WARNING: Warning in /home/vsts/work/1/s/src/hhalignment.cpp:3525: MergeMasterSlave:

  • 13:29:14.096 WARNING: maximum number of 65535 sequences exceeded while reading tr|A0A1B0CH20|A0A1B0CH20_LUTLO. Skipping all following sequences of this MSA

  • 13:29:14.097 WARNING: Warning in /home/vsts/work/1/s/src/hhalignment.cpp:3525: MergeMasterSlave:

  • 13:29:14.097 WARNING: maximum number of 65535 sequences exceeded while reading tr|A0A2P6N286|A0A2P6N286_9MYCE. Skipping all following sequences of this MSA

  • 13:29:14.097 WARNING: Warning in /home/vsts/work/1/s/src/hhalignment.cpp:3525: MergeMasterSlave:

  • 13:29:14.097 WARNING: maximum number of 65535 sequences exceeded while reading tr|A0A1Y2ER94|A0A1Y2ER94_9BASI. Skipping all following sequences of this MSA

  • 13:29:14.097 WARNING: Warning in /home/vsts/work/1/s/src/hhalignment.cpp:3525: MergeMasterSlave:

  • 13:29:14.097 WARNING: maximum number of 65535 sequences exceeded while reading tr|A0A1R2AT74|A0A1R2AT74_9CILI. Skipping all following sequences of this MSA

  • 13:29:14.097 WARNING: Warning in /home/vsts/work/1/s/src/hhalignment.cpp:3525: MergeMasterSlave:

  • 13:29:14.097 WARNING: maximum number of 65535 sequences exceeded while reading tr|A0A2V8MAI9|A0A2V8MAI9_9BACT. Skipping all following sequences of this MSA

  • 13:29:14.097 WARNING: Warning in /home/vsts/work/1/s/src/hhalignment.cpp:3525: MergeMasterSlave:

  • 13:29:14.097 WARNING: maximum number of 65535 sequences exceeded while reading tr|A0A118JZ66|A0A118JZ66_CYNCS. Skipping all following sequences of this MSA

  • 13:29:14.097 WARNING: Warning in /home/vsts/work/1/s/src/hhalignment.cpp:3525: MergeMasterSlave:

  • 13:29:14.097 WARNING: maximum number of 65535 sequences exceeded while reading tr|A0A2G2ZJB3|A0A2G2ZJB3_CAPAN. Skipping all following sequences of this MSA

  • 13:29:14.097 WARNING: Warning in /home/vsts/work/1/s/src/hhalignment.cpp:3525: MergeMasterSlave:

  • 13:29:14.097 WARNING: maximum number of 65535 sequences exceeded while reading tr|A0A0P4VPT4|A0A0P4VPT4_9EUCA. Skipping all following sequences of this MSA

  • 13:29:14.097 WARNING: Warning in /home/vsts/work/1/s/src/hhalignment.cpp:3525: MergeMasterSlave:

  • 13:29:14.097 WARNING: maximum number of 65535 sequences exceeded while reading tr|A0A0B6YY71|A0A0B6YY71_9EUPU. Skipping all following sequences of this MSA

  • 13:29:14.097 WARNING: Warning in /home/vsts/work/1/s/src/hhalignment.cpp:3525: MergeMasterSlave:

  • 13:29:14.097 WARNING: maximum number of 65535 sequences exceeded while reading tr|S9WHM1|S9WHM1_9TRYP. Skipping all following sequences of this MSA

  • 13:29:14.097 WARNING: Warning in /home/vsts/work/1/s/src/hhalignment.cpp:3525: MergeMasterSlave:

  • 13:29:14.097 WARNING: maximum number of 65535 sequences exceeded while reading tr|A0A0B3A2L0|A0A0B3A2L0_ARCG1. Skipping all following sequences of this MSA

  • 13:29:14.097 WARNING: Warning in /home/vsts/work/1/s/src/hhalignment.cpp:3525: MergeMasterSlave:

  • 13:29:14.097 WARNING: maximum number of 65535 sequences exceeded while reading tr|A0A1W9QC53|A0A1W9QC53_9DELT. Skipping all following sequences of this MSA

  • 13:29:14.097 WARNING: Warning in /home/vsts/work/1/s/src/hhalignment.cpp:3525: MergeMasterSlave:

  • 13:29:14.097 WARNING: maximum number of 65535 sequences exceeded while reading tr|A0A251V8P1|A0A251V8P1_HELAN. Skipping all following sequences of this MSA

  • 13:29:14.097 WARNING: Warning in /home/vsts/work/1/s/src/hhalignment.cpp:3525: MergeMasterSlave:

  • 13:29:14.097 WARNING: maximum number of 65535 sequences exceeded while reading tr|A0A154P6R7|A0A154P6R7_9HYME. Skipping all following sequences of this MSA

  • 13:29:14.097 WARNING: Warning in /home/vsts/work/1/s/src/hhalignment.cpp:3525: MergeMasterSlave:

  • 13:29:14.097 WARNING: maximum number of 65535 sequences exceeded while reading tr|E9EAV1|E9EAV1_METAQ. Skipping all following sequences of this MSA

  • 13:29:14.098 WARNING: Warning in /home/vsts/work/1/s/src/hhalignment.cpp:3525: MergeMasterSlave:

  • 13:29:14.098 WARNING: maximum number of 65535 sequences exceeded while reading tr|A0A151R1S2|A0A151R1S2_CAJCA. Skipping all following sequences of this MSA

  • 13:29:14.098 WARNING: Warning in /home/vsts/work/1/s/src/hhalignment.cpp:3525: MergeMasterSlave:

  • 13:29:14.098 WARNING: maximum number of 65535 sequences exceeded while reading tr|A0A0E9S388|A0A0E9S388_ANGAN. Skipping all following sequences of this MSA

  • 13:29:14.098 WARNING: Warning in /home/vsts/work/1/s/src/hhalignment.cpp:3525: MergeMasterSlave:

  • 13:29:14.098 WARNING: maximum number of 65535 sequences exceeded while reading tr|A0A225UWZ4|A0A225UWZ4_9STRA. Skipping all following sequences of this MSA

  • 13:29:14.098 WARNING: Warning in /home/vsts/work/1/s/src/hhalignment.cpp:3525: MergeMasterSlave:

  • 13:29:14.098 WARNING: maximum number of 65535 sequences exceeded while reading tr|A0A162SD93|A0A162SD93_9CRUS. Skipping all following sequences of this MSA

  • 13:29:14.098 WARNING: Warning in /home/vsts/work/1/s/src/hhalignment.cpp:3525: MergeMasterSlave:

  • 13:29:14.098 WARNING: maximum number of 65535 sequences exceeded while reading tr|G3I8Z3|G3I8Z3_CRIGR. Skipping all following sequences of this MSA

  • 13:29:14.098 WARNING: Warning in /home/vsts/work/1/s/src/hhalignment.cpp:3525: MergeMasterSlave:

  • 13:29:14.098 WARNING: maximum number of 65535 sequences exceeded while reading tr|A0A0N4UN36|A0A0N4UN36_DRAME. Skipping all following sequences of this MSA

  • 13:29:14.098 WARNING: Warning in /home/vsts/work/1/s/src/hhalignment.cpp:3525: MergeMasterSlave:

  • 13:29:14.098 WARNING: maximum number of 65535 sequences exceeded while reading tr|T0PX18|T0PX18_9STRA. Skipping all following sequences of this MSA

  • 13:29:14.099 WARNING: Warning in /home/vsts/work/1/s/src/hhalignment.cpp:3525: MergeMasterSlave:

  • 13:29:14.099 WARNING: maximum number of 65535 sequences exceeded while reading tr|A0A2J8M5N4|A0A2J8M5N4_PANTR. Skipping all following sequences of this MSA

  • 13:29:14.099 WARNING: Warning in /home/vsts/work/1/s/src/hhalignment.cpp:3525: MergeMasterSlave:

  • 13:29:14.099 WARNING: maximum number of 65535 sequences exceeded while reading tr|K3WDH8|K3WDH8_PYTUL. Skipping all following sequences of this MSA

  • 13:29:14.100 WARNING: Warning in /home/vsts/work/1/s/src/hhalignment.cpp:3525: MergeMasterSlave:

  • 13:29:14.100 WARNING: maximum number of 65535 sequences exceeded while reading tr|A0A2P2F0Z8|A0A2P2F0Z8_9EURO. Skipping all following sequences of this MSA

  • 13:29:14.100 WARNING: Warning in /home/vsts/work/1/s/src/hhalignment.cpp:3525: MergeMasterSlave:

  • 13:29:14.100 WARNING: maximum number of 65535 sequences exceeded while reading tr|A0A1A8AT91|A0A1A8AT91_NOTFU. Skipping all following sequences of this MSA

  • 13:29:14.100 WARNING: Warning in /home/vsts/work/1/s/src/hhalignment.cpp:3525: MergeMasterSlave:

  • 13:29:14.100 WARNING: maximum number of 65535 sequences exceeded while reading tr|A0A2A4FYH0|A0A2A4FYH0_9SPHN. Skipping all following sequences of this MSA

  • 13:29:14.100 WARNING: Warning in /home/vsts/work/1/s/src/hhalignment.cpp:3525: MergeMasterSlave:

  • 13:29:14.100 WARNING: maximum number of 65535 sequences exceeded while reading tr|Q233X3|Q233X3_TETTS. Skipping all following sequences of this MSA

  • 13:29:14.100 WARNING: Warning in /home/vsts/work/1/s/src/hhalignment.cpp:3525: MergeMasterSlave:

  • 13:29:14.100 WARNING: maximum number of 65535 sequences exceeded while reading tr|A0A0D7B0H2|A0A0D7B0H2_9AGAR. Skipping all following sequences of this MSA

  • 13:29:14.101 WARNING: Warning in /home/vsts/work/1/s/src/hhalignment.cpp:3525: MergeMasterSlave:

  • 13:29:14.101 WARNING: maximum number of 65535 sequences exceeded while reading tr|V3ZEY5|V3ZEY5_LOTGI. Skipping all following sequences of this MSA

  • 13:29:14.101 WARNING: Warning in /home/vsts/work/1/s/src/hhalignment.cpp:3525: MergeMasterSlave:

  • 13:29:14.101 WARNING: maximum number of 65535 sequences exceeded while reading tr|A0A2U1NK47|A0A2U1NK47_ARTAN. Skipping all following sequences of this MSA

  • 13:29:14.101 WARNING: Warning in /home/vsts/work/1/s/src/hhalignment.cpp:3525: MergeMasterSlave:

  • 13:29:14.101 WARNING: maximum number of 65535 sequences exceeded while reading tr|A0A0D2MMR0|A0A0D2MMR0_9CHLO. Skipping all following sequences of this MSA

  • 13:29:14.101 WARNING: Warning in /home/vsts/work/1/s/src/hhalignment.cpp:3525: MergeMasterSlave:

  • 13:29:14.101 WARNING: maximum number of 65535 sequences exceeded while reading tr|C9ZKY9|C9ZKY9_TRYB9. Skipping all following sequences of this MSA

  • 13:29:14.103 WARNING: Warning in /home/vsts/work/1/s/src/hhalignment.cpp:3525: MergeMasterSlave:

  • 13:29:14.103 WARNING: maximum number of 65535 sequences exceeded while reading tr|A0A0K9RN00|A0A0K9RN00_SPIOL. Skipping all following sequences of this MSA

  • 13:29:14.105 WARNING: Warning in /home/vsts/work/1/s/src/hhalignment.cpp:3525: MergeMasterSlave:

  • 13:29:14.105 WARNING: maximum number of 65535 sequences exceeded while reading tr|A0A194WW17|A0A194WW17_9HELO. Skipping all following sequences of this MSA

  • 13:29:14.105 WARNING: Warning in /home/vsts/work/1/s/src/hhalignment.cpp:3525: MergeMasterSlave:

  • 13:29:14.105 WARNING: maximum number of 65535 sequences exceeded while reading tr|A0A0K0FAK9|A0A0K0FAK9_9BILA. Skipping all following sequences of this MSA

  • 13:29:14.105 WARNING: Warning in /home/vsts/work/1/s/src/hhalignment.cpp:3525: MergeMasterSlave:

  • 13:29:14.105 WARNING: maximum number of 65535 sequences exceeded while reading tr|A0A1I8CB81|A0A1I8CB81_9BILA. Skipping all following sequences of this MSA

  • 13:29:14.105 WARNING: Warning in /home/vsts/work/1/s/src/hhalignment.cpp:3525: MergeMasterSlave:

  • 13:29:14.105 WARNING: maximum number of 65535 sequences exceeded while reading tr|A0A1I7U9Q8|A0A1I7U9Q8_9PELO. Skipping all following sequences of this MSA

  • 13:29:14.105 WARNING: Warning in /home/vsts/work/1/s/src/hhalignment.cpp:3525: MergeMasterSlave:

  • 13:29:14.106 WARNING: maximum number of 65535 sequences exceeded while reading tr|M5WN03|M5WN03_PRUPE. Skipping all following sequences of this MSA

  • 13:29:14.106 WARNING: Warning in /home/vsts/work/1/s/src/hhalignment.cpp:3525: MergeMasterSlave:

  • 13:29:14.106 WARNING: maximum number of 65535 sequences exceeded while reading tr|A0A2P5I978|A0A2P5I978_9PEZI. Skipping all following sequences of this MSA

  • 13:29:14.106 WARNING: Warning in /home/vsts/work/1/s/src/hhalignment.cpp:3525: MergeMasterSlave:

  • 13:29:14.106 WARNING: maximum number of 65535 sequences exceeded while reading tr|A0A2G5CE51|A0A2G5CE51_AQUCA. Skipping all following sequences of this MSA

  • 13:29:14.106 WARNING: Warning in /home/vsts/work/1/s/src/hhalignment.cpp:3525: MergeMasterSlave:

  • 13:29:14.106 WARNING: maximum number of 65535 sequences exceeded while reading tr|T1HCH7|T1HCH7_RHOPR. Skipping all following sequences of this MSA

  • 13:29:14.106 WARNING: Warning in /home/vsts/work/1/s/src/hhalignment.cpp:3525: MergeMasterSlave:

  • 13:29:14.106 WARNING: maximum number of 65535 sequences exceeded while reading tr|A0A0G4NDC1|A0A0G4NDC1_9PEZI. Skipping all following sequences of this MSA

  • 13:29:14.106 WARNING: Warning in /home/vsts/work/1/s/src/hhalignment.cpp:3525: MergeMasterSlave:

  • 13:29:14.106 WARNING: maximum number of 65535 sequences exceeded while reading tr|A0A2I4BLW5|A0A2I4BLW5_9TELE. Skipping all following sequences of this MSA

  • 13:29:14.106 WARNING: Warning in /home/vsts/work/1/s/src/hhalignment.cpp:3525: MergeMasterSlave:

  • 13:29:14.106 WARNING: maximum number of 65535 sequences exceeded while reading tr|A0A061EL09|A0A061EL09_THECC. Skipping all following sequences of this MSA

  • 13:29:14.106 WARNING: Warning in /home/vsts/work/1/s/src/hhalignment.cpp:3525: MergeMasterSlave:

  • 13:29:14.106 WARNING: maximum number of 65535 sequences exceeded while reading tr|A0A2L2TZW0|A0A2L2TZW0_9HYPO. Skipping all following sequences of this MSA

  • 13:29:14.106 WARNING: Warning in /home/vsts/work/1/s/src/hhalignment.cpp:3525: MergeMasterSlave:

  • 13:29:14.106 WARNING: maximum number of 65535 sequences exceeded while reading tr|A0A182FWA4|A0A182FWA4_ANOAL. Skipping all following sequences of this MSA

  • 13:29:14.106 WARNING: Warning in /home/vsts/work/1/s/src/hhalignment.cpp:3525: MergeMasterSlave:

  • 13:29:14.106 WARNING: maximum number of 65535 sequences exceeded while reading tr|A0A167FTS4|A0A167FTS4_9BASI. Skipping all following sequences of this MSA

  • 13:29:14.106 WARNING: Warning in /home/vsts/work/1/s/src/hhalignment.cpp:3525: MergeMasterSlave:

  • 13:29:14.106 WARNING: maximum number of 65535 sequences exceeded while reading tr|D8UGB6|D8UGB6_VOLCA. Skipping all following sequences of this MSA

  • 13:29:14.106 WARNING: Warning in /home/vsts/work/1/s/src/hhalignment.cpp:3525: MergeMasterSlave:

  • 13:29:14.106 WARNING: maximum number of 65535 sequences exceeded while reading tr|T0SI96|T0SI96_9STRA. Skipping all following sequences of this MSA

  • 13:29:14.106 WARNING: Warning in /home/vsts/work/1/s/src/hhalignment.cpp:3525: MergeMasterSlave:

  • 13:29:14.106 WARNING: maximum number of 65535 sequences exceeded while reading tr|A0A183EZN9|A0A183EZN9_9BILA. Skipping all following sequences of this MSA

  • 13:29:14.106 WARNING: Warning in /home/vsts/work/1/s/src/hhalignment.cpp:3525: MergeMasterSlave:

  • 13:29:14.106 WARNING: maximum number of 65535 sequences exceeded while reading tr|Q6BZI9|Q6BZI9_DEBHA. Skipping all following sequences of this MSA

  • 13:29:14.106 WARNING: Warning in /home/vsts/work/1/s/src/hhalignment.cpp:3525: MergeMasterSlave:

  • 13:29:14.106 WARNING: maximum number of 65535 sequences exceeded while reading tr|A0A0R3S8Q1|A0A0R3S8Q1_HYMDI. Skipping all following sequences of this MSA

  • 13:29:14.107 WARNING: Warning in /home/vsts/work/1/s/src/hhalignment.cpp:3525: MergeMasterSlave:

  • 13:29:14.107 WARNING: maximum number of 65535 sequences exceeded while reading tr|K1VWU8|K1VWU8_TRIAC. Skipping all following sequences of this MSA

  • 13:29:14.107 WARNING: Warning in /home/vsts/work/1/s/src/hhalignment.cpp:3525: MergeMasterSlave:

  • 13:29:14.107 WARNING: maximum number of 65535 sequences exceeded while reading tr|W8B0A1|W8B0A1_CERCA. Skipping all following sequences of this MSA

  • 13:29:14.107 WARNING: Warning in /home/vsts/work/1/s/src/hhalignment.cpp:3525: MergeMasterSlave:

  • 13:29:14.107 WARNING: maximum number of 65535 sequences exceeded while reading tr|X1TU32|X1TU32_9ZZZZ. Skipping all following sequences of this MSA

  • 13:29:14.107 WARNING: Warning in /home/vsts/work/1/s/src/hhalignment.cpp:3525: MergeMasterSlave:

  • 13:29:14.107 WARNING: maximum number of 65535 sequences exceeded while reading tr|A0A067FBF0|A0A067FBF0_CITSI. Skipping all following sequences of this MSA

  • 13:29:14.107 WARNING: Warning in /home/vsts/work/1/s/src/hhalignment.cpp:3525: MergeMasterSlave:

  • 13:29:14.107 WARNING: maximum number of 65535 sequences exceeded while reading tr|U9UP77|U9UP77_RHIID. Skipping all following sequences of this MSA

  • 13:29:14.107 WARNING: Warning in /home/vsts/work/1/s/src/hhalignment.cpp:3525: MergeMasterSlave:

  • 13:29:14.107 WARNING: maximum number of 65535 sequences exceeded while reading tr|T1EQC7|T1EQC7_HELRO. Skipping all following sequences of this MSA

  • 13:29:14.107 WARNING: Warning in /home/vsts/work/1/s/src/hhalignment.cpp:3525: MergeMasterSlave:

  • 13:29:14.107 WARNING: maximum number of 65535 sequences exceeded while reading tr|A0A0K0DFC3|A0A0K0DFC3_ANGCA. Skipping all following sequences of this MSA

  • 13:29:14.107 WARNING: Warning in /home/vsts/work/1/s/src/hhalignment.cpp:3525: MergeMasterSlave:

  • 13:29:14.107 WARNING: maximum number of 65535 sequences exceeded while reading tr|A0A226D6M1|A0A226D6M1_FOLCA. Skipping all following sequences of this MSA

  • 13:29:14.107 WARNING: Warning in /home/vsts/work/1/s/src/hhalignment.cpp:3525: MergeMasterSlave:

  • 13:29:14.107 WARNING: maximum number of 65535 sequences exceeded while reading tr|A0A091KUC1|A0A091KUC1_COLST. Skipping all following sequences of this MSA

  • 13:29:14.107 WARNING: Warning in /home/vsts/work/1/s/src/hhalignment.cpp:3525: MergeMasterSlave:

  • 13:29:14.107 WARNING: maximum number of 65535 sequences exceeded while reading tr|A0A2V9CFX3|A0A2V9CFX3_9BACT. Skipping all following sequences of this MSA

  • 13:29:14.109 WARNING: Warning in /home/vsts/work/1/s/src/hhalignment.cpp:3525: MergeMasterSlave:

  • 13:29:14.109 WARNING: maximum number of 65535 sequences exceeded while reading tr|A0A287GNT2|A0A287GNT2_HORVV. Skipping all following sequences of this MSA

  • 13:29:14.109 WARNING: Warning in /home/vsts/work/1/s/src/hhalignment.cpp:3525: MergeMasterSlave:

  • 13:29:14.109 WARNING: maximum number of 65535 sequences exceeded while reading tr|A0A0K9R7H3|A0A0K9R7H3_SPIOL. Skipping all following sequences of this MSA

  • 13:29:14.109 WARNING: Warning in /home/vsts/work/1/s/src/hhalignment.cpp:3525: MergeMasterSlave:

  • 13:29:14.109 WARNING: maximum number of 65535 sequences exceeded while reading tr|A0A2H3XKB4|A0A2H3XKB4_PHODC. Skipping all following sequences of this MSA

  • 13:29:14.109 WARNING: Warning in /home/vsts/work/1/s/src/hhalignment.cpp:3525: MergeMasterSlave:

  • 13:29:14.109 WARNING: maximum number of 65535 sequences exceeded while reading tr|A0A1G0ICC9|A0A1G0ICC9_9GAMM. Skipping all following sequences of this MSA

  • 13:29:14.109 WARNING: Warning in /home/vsts/work/1/s/src/hhalignment.cpp:3525: MergeMasterSlave:

  • 13:29:14.109 WARNING: maximum number of 65535 sequences exceeded while reading tr|K0KKA1|K0KKA1_WICCF. Skipping all following sequences of this MSA

  • 13:29:14.109 WARNING: Warning in /home/vsts/work/1/s/src/hhalignment.cpp:3525: MergeMasterSlave:

  • 13:29:14.109 WARNING: maximum number of 65535 sequences exceeded while reading tr|A0A146K5D2|A0A146K5D2_9EUKA. Skipping all following sequences of this MSA

  • 13:29:14.109 WARNING: Warning in /home/vsts/work/1/s/src/hhalignment.cpp:3525: MergeMasterSlave:

  • 13:29:14.109 WARNING: maximum number of 65535 sequences exceeded while reading tr|A0A196SA88|A0A196SA88_BLAHN. Skipping all following sequences of this MSA

  • 13:29:14.109 WARNING: Warning in /home/vsts/work/1/s/src/hhalignment.cpp:3525: MergeMasterSlave:

  • 13:29:14.109 WARNING: maximum number of 65535 sequences exceeded while reading tr|A0A0D9WQJ3|A0A0D9WQJ3_9ORYZ. Skipping all following sequences of this MSA

  • 13:29:14.110 WARNING: Warning in /home/vsts/work/1/s/src/hhalignment.cpp:3525: MergeMasterSlave:

  • 13:29:14.110 WARNING: maximum number of 65535 sequences exceeded while reading tr|A0A1I8JS06|A0A1I8JS06_9PLAT. Skipping all following sequences of this MSA

  • 13:29:14.110 WARNING: Warning in /home/vsts/work/1/s/src/hhalignment.cpp:3525: MergeMasterSlave:

  • 13:29:14.110 WARNING: maximum number of 65535 sequences exceeded while reading tr|A0A2E1MYT9|A0A2E1MYT9_9BACT. Skipping all following sequences of this MSA

  • 13:29:14.110 WARNING: Warning in /home/vsts/work/1/s/src/hhalignment.cpp:3525: MergeMasterSlave:

  • 13:29:14.110 WARNING: maximum number of 65535 sequences exceeded while reading tr|U9SJ25|U9SJ25_RHIID. Skipping all following sequences of this MSA

  • 13:29:14.110 WARNING: Warning in /home/vsts/work/1/s/src/hhalignment.cpp:3525: MergeMasterSlave:

  • 13:29:14.110 WARNING: maximum number of 65535 sequences exceeded while reading tr|A0A1D6MQR8|A0A1D6MQR8_MAIZE. Skipping all following sequences of this MSA

  • 13:29:14.110 WARNING: Warning in /home/vsts/work/1/s/src/hhalignment.cpp:3525: MergeMasterSlave:

  • 13:29:14.110 WARNING: maximum number of 65535 sequences exceeded while reading tr|A0A254WLY3|A0A254WLY3_9CYAN. Skipping all following sequences of this MSA

  • 13:29:14.110 WARNING: Warning in /home/vsts/work/1/s/src/hhalignment.cpp:3525: MergeMasterSlave:

  • 13:29:14.110 WARNING: maximum number of 65535 sequences exceeded while reading tr|A0A2K3K9K2|A0A2K3K9K2_TRIPR. Skipping all following sequences of this MSA

  • 13:29:14.110 WARNING: Warning in /home/vsts/work/1/s/src/hhalignment.cpp:3525: MergeMasterSlave:

  • 13:29:14.110 WARNING: maximum number of 65535 sequences exceeded while reading tr|A0A1R3J7P9|A0A1R3J7P9_9ROSI. Skipping all following sequences of this MSA

  • 13:29:14.110 WARNING: Warning in /home/vsts/work/1/s/src/hhalignment.cpp:3525: MergeMasterSlave:

  • 13:29:14.110 WARNING: maximum number of 65535 sequences exceeded while reading tr|A0A1Y5FT88|A0A1Y5FT88_9GAMM. Skipping all following sequences of this MSA

  • 13:29:14.110 WARNING: Warning in /home/vsts/work/1/s/src/hhalignment.cpp:3525: MergeMasterSlave:

  • 13:29:14.110 WARNING: maximum number of 65535 sequences exceeded while reading tr|A0A0M9F0X5|A0A0M9F0X5_FUSLA. Skipping all following sequences of this MSA

  • 13:29:14.110 WARNING: Warning in /home/vsts/work/1/s/src/hhalignment.cpp:3525: MergeMasterSlave:

  • 13:29:14.110 WARNING: maximum number of 65535 sequences exceeded while reading tr|A0A0D7AN08|A0A0D7AN08_9AGAR. Skipping all following sequences of this MSA

  • 13:29:14.111 WARNING: Warning in /home/vsts/work/1/s/src/hhalignment.cpp:3525: MergeMasterSlave:

  • 13:29:14.111 WARNING: maximum number of 65535 sequences exceeded while reading tr|A0A0C9SKA2|A0A0C9SKA2_PLICR. Skipping all following sequences of this MSA

  • 13:29:14.111 WARNING: Warning in /home/vsts/work/1/s/src/hhalignment.cpp:3525: MergeMasterSlave:

  • 13:29:14.111 WARNING: maximum number of 65535 sequences exceeded while reading tr|A0A2V8KNU2|A0A2V8KNU2_9BACT. Skipping all following sequences of this MSA

  • 13:29:14.111 WARNING: Warning in /home/vsts/work/1/s/src/hhalignment.cpp:3525: MergeMasterSlave:

  • 13:29:14.111 WARNING: maximum number of 65535 sequences exceeded while reading tr|D7FM30|D7FM30_ECTSI. Skipping all following sequences of this MSA

  • 13:29:14.111 WARNING: Warning in /home/vsts/work/1/s/src/hhalignment.cpp:3525: MergeMasterSlave:

  • 13:29:14.111 WARNING: maximum number of 65535 sequences exceeded while reading tr|A0A2H3AZD9|A0A2H3AZD9_9AGAR. Skipping all following sequences of this MSA

  • 13:29:14.111 WARNING: Warning in /home/vsts/work/1/s/src/hhalignment.cpp:3525: MergeMasterSlave:

  • 13:29:14.111 WARNING: maximum number of 65535 sequences exceeded while reading tr|J4I2U5|J4I2U5_9APHY. Skipping all following sequences of this MSA

  • 13:29:14.115 WARNING: Warning in /home/vsts/work/1/s/src/hhalignment.cpp:3525: MergeMasterSlave:

  • 13:29:14.115 WARNING: maximum number of 65535 sequences exceeded while reading tr|A0A2R6DCV8|A0A2R6DCV8_9EURY. Skipping all following sequences of this MSA

  • 13:29:14.123 WARNING: Warning in /home/vsts/work/1/s/src/hhalignment.cpp:3525: MergeMasterSlave:

  • 13:29:14.123 WARNING: maximum number of 65535 sequences exceeded while reading tr|A0A2P4HNQ7|A0A2P4HNQ7_QUESU. Skipping all following sequences of this MSA

  • 13:29:14.123 WARNING: Warning in /home/vsts/work/1/s/src/hhalignment.cpp:3525: MergeMasterSlave:

  • 13:29:14.123 WARNING: maximum number of 65535 sequences exceeded while reading tr|A0A1G0J1C4|A0A1G0J1C4_9GAMM. Skipping all following sequences of this MSA

  • 13:29:14.123 WARNING: Warning in /home/vsts/work/1/s/src/hhalignment.cpp:3525: MergeMasterSlave:

  • 13:29:14.123 WARNING: maximum number of 65535 sequences exceeded while reading tr|A0A1V4YX42|A0A1V4YX42_9EURY. Skipping all following sequences of this MSA

  • 13:29:14.123 WARNING: Warning in /home/vsts/work/1/s/src/hhalignment.cpp:3525: MergeMasterSlave:

  • 13:29:14.123 WARNING: maximum number of 65535 sequences exceeded while reading tr|A0A077ZTA0|A0A077ZTA0_STYLE. Skipping all following sequences of this MSA

  • 13:29:14.124 WARNING: Warning in /home/vsts/work/1/s/src/hhalignment.cpp:3525: MergeMasterSlave:

  • 13:29:14.124 WARNING: maximum number of 65535 sequences exceeded while reading tr|A0A2E8M8X6|A0A2E8M8X6_9BACT. Skipping all following sequences of this MSA

  • 13:29:14.125 WARNING: Warning in /home/vsts/work/1/s/src/hhalignment.cpp:3525: MergeMasterSlave:

  • 13:29:14.125 WARNING: maximum number of 65535 sequences exceeded while reading tr|A0A2H5QA60|A0A2H5QA60_CITUN. Skipping all following sequences of this MSA

  • 13:29:14.125 WARNING: Warning in /home/vsts/work/1/s/src/hhalignment.cpp:3525: MergeMasterSlave:

  • 13:29:14.125 WARNING: maximum number of 65535 sequences exceeded while reading tr|A0A2G6I5D1|A0A2G6I5D1_SORCE. Skipping all following sequences of this MSA

  • 13:29:14.145 WARNING: Warning in /home/vsts/work/1/s/src/hhalignment.cpp:3525: MergeMasterSlave:

  • 13:29:14.145 WARNING: maximum number of 65535 sequences exceeded while reading tr|A0A022RZI6|A0A022RZI6_ERYGU. Skipping all following sequences of this MSA

  • 13:29:14.153 WARNING: Warning in /home/vsts/work/1/s/src/hhalignment.cpp:3525: MergeMasterSlave:

  • 13:29:14.153 WARNING: maximum number of 65535 sequences exceeded while reading tr|A0A137QLC5|A0A137QLC5_9AGAR. Skipping all following sequences of this MSA

  • 13:29:14.154 WARNING: Warning in /home/vsts/work/1/s/src/hhalignment.cpp:3525: MergeMasterSlave:

  • 13:29:14.154 WARNING: maximum number of 65535 sequences exceeded while reading tr|S7TFY7|S7TFY7_9DELT. Skipping all following sequences of this MSA

  • 13:29:14.156 WARNING: Warning in /home/vsts/work/1/s/src/hhalignment.cpp:3525: MergeMasterSlave:

  • 13:29:14.156 WARNING: maximum number of 65535 sequences exceeded while reading tr|A0A229XDM0|A0A229XDM0_ASPFM. Skipping all following sequences of this MSA

  • 13:29:14.157 WARNING: Warning in /home/vsts/work/1/s/src/hhalignment.cpp:3525: MergeMasterSlave:

  • 13:29:14.157 WARNING: maximum number of 65535 sequences exceeded while reading tr|A0A077B9S0|A0A077B9S0_9ALPH. Skipping all following sequences of this MSA

  • 13:29:14.157 WARNING: Warning in /home/vsts/work/1/s/src/hhalignment.cpp:3525: MergeMasterSlave:

  • 13:29:14.157 WARNING: maximum number of 65535 sequences exceeded while reading tr|A0A2I1HNA4|A0A2I1HNA4_9GLOM. Skipping all following sequences of this MSA

  • 13:29:14.158 WARNING: Warning in /home/vsts/work/1/s/src/hhalignment.cpp:3525: MergeMasterSlave:

  • 13:29:14.158 WARNING: maximum number of 65535 sequences exceeded while reading tr|A0A089ZKD9|A0A089ZKD9_9LACO. Skipping all following sequences of this MSA

  • 13:29:14.162 WARNING: Warning in /home/vsts/work/1/s/src/hhalignment.cpp:3525: MergeMasterSlave:

  • 13:29:14.162 WARNING: maximum number of 65535 sequences exceeded while reading tr|A0A167D423|A0A167D423_9GAMM. Skipping all following sequences of this MSA

  • 13:29:23.938 INFO: 77344 sequences belonging to 77344 database HMMs found with an E-value < 0.001

  • 13:29:23.938 INFO: Number of effective sequences of resulting query HMM: Neff = 5.47474

  • 13:29:28.042 INFO: Iteration 3

  • 13:29:28.241 INFO: Prefiltering database

  • 13:29:46.041 INFO: HMMs passed 1st prefilter (gapless profile-profile alignment) : 2535107

  • 13:30:03.580 WARNING: Number of hits passing 2nd prefilter (reduced from 292702 to allowed maximum of 100000). You can increase the allowed maximum using the -maxfilt option.

  • 13:30:03.747 INFO: HMMs passed 2nd prefilter (gapped profile-profile alignment) : 100000

  • 13:30:03.747 INFO: HMMs passed 2nd prefilter and not found in previous iterations : 42561

  • 13:30:03.747 INFO: Scoring 42561 HMMs using HMM-HMM Viterbi alignment

  • 13:30:03.814 INFO: Alternative alignment: 0

  • 13:30:04.920 INFO: 2000 alignments done

  • 13:30:06.045 INFO: 4000 alignments done

  • 13:30:07.139 INFO: 6000 alignments done

zj223332 avatar Feb 19 '24 05:02 zj223332