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using ensemblgenomes for non-vertebrate references

Open RvV1979 opened this issue 3 years ago • 2 comments

Hi Johannes, I am trying to implement the https://github.com/snakemake-workflows/dna-seq-gatk-variant-calling workflow for non-vertebrate (plant) genomes that are available through ensemblgenomes.org. However, the https://github.com/snakemake/snakemake-wrappers/tree/master/bio/reference wrappers use only the canonical ensembl ftp site.

Is it possible to use these wrappers for ensemblgenomes ?

Thanks

RvV1979 avatar Apr 17 '21 08:04 RvV1979

The wrapper currently uses the URL ftp://ftp.ensembl.org/pub/{branch}release-{release}/fasta/{species}/{datatype}/{species_cap}.{spec}.{suffix}.

For ensembl genomes, would it be enough to change it to ftp://ftp.{source}.org/pub/{branch}release-{release}/fasta/{species}/{datatype}/{species_cap}.{spec}.{suffix}?

Can you provide an example snakemake rule for testing?

fgvieira avatar Apr 01 '22 07:04 fgvieira

I believe this has been fixed on #546. Can you double check?

fgvieira avatar Aug 22 '22 07:08 fgvieira