Whether the spatial coordinates of integrated SRT data from multiple samples influence downstream analyses?

[jianghao0799]

I have 9 samples, with three stages and three replicates at each stage. And I use merge() function from seurat to merge 9 SRT data. I have completed the basic analysis.

Spatial embedding like this:

p <- DimPlot(
  sce.big, 
  split.by='leiden_cluster',
  reduction = 'spatial',
  ncol=4
) + NoLegend() + umap_theme()
p

Does the proximity of spatial distance affect the construction?