Implement TMT quantification, including quantifying protein groups

[giladwv]

trafficstars

Maybe I missed it. Can one use TMT quantification with metamorpheous?

[trishorts]

Unfortunately, not at this time. You can do a search of TMT modified peptides by selecting the appropriate TMT labels from the Multiplex Label Checkbox uner Modifications. You have to click the triangle to open up and see all of the available options.

the reporter intensity will be provided in the output and you can analyze the results manually.

Adding TMT quant is a priority for me. You can follow this issue for updates. https://github.com/smith-chem-wisc/MetaMorpheus/issues/1935

[giladwv]

Thanks... But i need it on the protein groups. :)

I appreciate the swift answer. Best. G

Best, Gilad W. Vainer, M.D. Ph.D. Department of Pathology Hadassah Hebrew-University Medical Center Jerusalem, Israel

On Sun, Mar 14, 2021, 18:38 trishorts @.***> wrote:

Unfortunately, not at this time. You can do a search of TMT modified peptides by selecting the appropriate TMT labels from the Multiplex Label Checkbox uner Modifications. You have to click the triangle to open up and see all of the available options. [image: image] https://user-images.githubusercontent.com/16841846/111076228-79127100-84b9-11eb-8e58-c640666f0d77.png

[image: image] https://user-images.githubusercontent.com/16841846/111076246-8891ba00-84b9-11eb-9fed-6fb67891cb3b.png

the reporter intensity will be provided in the output and you can analyze the results manually.

Adding TMT quant is a priority for me. You can follow this issue for updates. #1935 https://github.com/smith-chem-wisc/MetaMorpheus/issues/1935

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[acesnik]

See also https://github.com/smith-chem-wisc/MetaMorpheus/issues/1935 for more discussion.

• How to find rough TMT intensities at peptide level
• Could implement PIF like MaxQuant for label intensities at the peptide level to improve filtering the rough quant results.

[acesnik]

I want to bump this one, since TMT is popular and growing more popular.