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featureCounts subprocess.CalledProcessError

Open CaiCheng1996 opened this issue 9 months ago • 1 comments

subprocess.CalledProcessError: Command 'featureCounts -s 1 -a merged.combined.gtf -o ./La-alveolar-mid -R BAM -T 12 -t gene ./La-alveolar-mid/03.star/La-alveolar-mid_Aligned.sortedByCoord.out.bam 2>&1 ' returned non-zero exit status 255. Empty self.gene_id. Run self.get_id_name first.

Here is my gtf: Contig1 . transcript 554626 556699 . + . gene_id "XLOC.000001"; transcript_id "TCONS.00000001"; Contig1 . exon 554626 556699 . + . gene_id "XLOC.000001"; transcript_id "TCONS.00000001"; Contig1 EVM transcript 666201 666896 . - . gene_id "XLOC.000002"; transcript_id "TCONS.00000002"; Contig1 EVM exon 666201 666896 . - . gene_id "XLOC.000002"; transcript_id "TCONS.00000002"; Contig10 EVM transcript 1438 18151 . - . gene_id "XLOC.000003"; transcript_id "TCONS.00000003"; Contig10 EVM exon 1438 1536 . - . gene_id "XLOC.000003"; transcript_id "TCONS.00000003"; Contig10 EVM exon 1649 1757 . - . gene_id "XLOC.000003"; transcript_id "TCONS.00000003"; Contig10 EVM exon 4068 4165 . - . gene_id "XLOC.000003"; transcript_id "TCONS.00000003"; Contig10 EVM exon 17951 18151 . - . gene_id "XLOC.000003"; transcript_id "TCONS.00000003";

CaiCheng1996 avatar May 29 '24 11:05 CaiCheng1996