Impossible to install Pfam to target location

[SilasK]

Sorry, I'm frustrated after some time debugging Virsorter.

Virsorter depends on a outdated version of Pfam. I can download it but apparently Virsorter requires it to be at /Pfam_27/which I don't have acces.

By the way: MGA can be installed using conda but needs to be renamed.

[simroux]

Hi Silas, Are you trying to run VirSorter through the Docker container or via cloning from GitHub + custom Conda environment ? If the latter, there is an option called "--data-dir" where you can provide a path to a folder including PFAM. This should look something like:

./wrapper_phage_contigs_sorter_iPlant.pl ... your other options ... --data-dir /path/to/dbs/

Then in the folder /path/to/dbs/ VirSorter will expect:

• a folder "PFAM_27/" with two files, Pfam-A.hmm and Pfam-B.hmm
• the files and folders from "virsorter-data-v2.tar.gz"

[SilasK]

Yea I use virsorter in a conda environment, I specified the datadir where I downloaded the vrsorter data v2.

If I understand the error message correctly virsorter was looking for /Pfam_27 (root/Pfam_27) which is only possible in the docker.

[simroux]

The error message is weird, and doesn't seem to be accurate (sorry). VirSorter expects Pfam to be in the same directory as the virsorter data v2, in its own subfolder called "PFAM_27". Could you test this and check if it works better ?