how_are_we_stranded_here
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Can't find any of the first 10 BED transcript_ids in fasta file
Hi,
I am getting the
Can't find any of the first 10 BED transcript_ids in fasta file
error while the transcript_ids of the bed file are clearly in the transcript fasta file.
I parsed the .gtf file with 'agat_convert_sp_gff2gtf.pl' and made a custom bash script to retain only the transcript_id as the FASTA header so there are no issues. Here is a sample of the gtf, the bed and the fasta files: GTF:
#!genome-build DOE Joint Genome Institute Ccitriodora_v2_1
#!genome-version Ccitriodora_v2_1
#!genome-date 2020-10
#!genome-build-accession GCA_014858505.1
#!genebuild-last-updated 2021-09
1 Ccitriodora_v2_1 region 1 31549113 . . . gene_id "region:1"; Alias "CM026410.1"; ID "region:1";
1 JGI gene 17519 18454 . + . gene_id "gene-BT93_A0001"; ID "gene:gene-BT93_A0001"; biotype "protein_coding"; logic_name "genemodel_jgi";
1 JGI mRNA 17519 18454 . + . gene_id "gene-BT93_A0001"; transcript_id "rna-gnl|WGS:JABURB|Cocit.A0001.1"; ID "transcript:rna-gnl|WGS:JABURB|Cocit.A0001.1"; Parent "gene:gene-BT93_A0001"; biotype "protein_coding"; tag "Ensembl_canonical";
1 JGI exon 17519 17859 . + . gene_id "gene-BT93_A0001"; transcript_id "rna-gnl|WGS:JABURB|Cocit.A0001.1"; ID "rna-gnl|WGS:JABURB|Cocit.A0001.1-E1"; Name "rna-gnl|WGS:JABURB|Cocit.A0001.1-E1"; Parent "transcript:rna-gnl|WGS:JABURB|Cocit.A0001.1"; constitutive "1"; ensembl_end_phase "2"; ensembl_phase "0"; exon_id "rna-gnl|WGS:JABURB|Cocit.A0001.1-E1"; rank "1";
1 JGI exon 17879 17949 . + . gene_id "gene-BT93_A0001"; transcript_id "rna-gnl|WGS:JABURB|Cocit.A0001.1"; ID "rna-gnl|WGS:JABURB|Cocit.A0001.1-E2"; Name "rna-gnl|WGS:JABURB|Cocit.A0001.1-E2"; Parent "transcript:rna-gnl|WGS:JABURB|Cocit.A0001.1"; constitutive "1"; ensembl_end_phase "1"; ensembl_phase "2"; exon_id "rna-gnl|WGS:JABURB|Cocit.A0001.1-E2"; rank "2";
1 JGI exon 18051 18454 . + . gene_id "gene-BT93_A0001"; transcript_id "rna-gnl|WGS:JABURB|Cocit.A0001.1"; ID "rna-gnl|WGS:JABURB|Cocit.A0001.1-E3"; Name "rna-gnl|WGS:JABURB|Cocit.A0001.1-E3"; Parent "transcript:rna-gnl|WGS:JABURB|Cocit.A0001.1"; constitutive "1"; ensembl_end_phase "0"; ensembl_phase "1"; exon_id "rna-gnl|WGS:JABURB|Cocit.A0001.1-E3"; rank "3";
1 JGI CDS 17519 17859 . + 0 gene_id "gene-BT93_A0001"; transcript_id "rna-gnl|WGS:JABURB|Cocit.A0001.1"; ID "CDS:cds-KAF8041257.1"; Parent "transcript:rna-gnl|WGS:JABURB|Cocit.A0001.1"; protein_id "cds-KAF8041257.1";
1 JGI CDS 17879 17949 . + 1 gene_id "gene-BT93_A0001"; transcript_id "rna-gnl|WGS:JABURB|Cocit.A0001.1"; ID "CDS:cds-KAF8041257.1"; Parent "transcript:rna-gnl|WGS:JABURB|Cocit.A0001.1"; protein_id "cds-KAF8041257.1";
1 JGI CDS 18051 18454 . + 2 gene_id "gene-BT93_A0001"; transcript_id "rna-gnl|WGS:JABURB|Cocit.A0001.1"; ID "CDS:cds-KAF8041257.1"; Parent "transcript:rna-gnl|WGS:JABURB|Cocit.A0001.1"; protein_id "cds-KAF8041257.1";
BED:
1 17518 18454 rna-gnl|WGS:JABURB|Cocit.A0001.1 0 + 17518 18454 0 3 341,71,404, 0,360,532,
1 31589 32512 rna-gnl|WGS:JABURB|Cocit.A0002.1 0 + 31589 32512 0 2 415,407, 0,516,
1 46163 47204 rna-gnl|WGS:JABURB|Cocit.A0003.1 0 + 46163 47204 0 2 441,475, 0,566,
1 62155 63760 rna-gnl|WGS:JABURB|Cocit.A0004.1 0 + 62155 63760 0 2 421,407, 0,1198,
1 64048 68743 rna-gnl|WGS:JABURB|Cocit.A0005.1 0 - 64048 68743 0 5 352,116,158,74,1541, 0,1195,1836,2993,3154,
1 70379 70571 rna-gnl|WGS:JABURB|Cocit.A0006.1 0 - 70379 70571 0 1 192, 0,
1 76482 79181 rna-gnl|WGS:JABURB|Cocit.A0007.1 0 + 76482 79181 0 3 360,387,570, 0,1132,2129,
1 76482 79181 rna-gnl|WGS:JABURB|Cocit.A0007.2 0 + 76482 79181 0 3 360,309,570, 0,1132,2129,
1 79626 88276 rna-gnl|WGS:JABURB|Cocit.A0008.1 0 - 79626 88276 0 10 551,102,86,272,92,138,71,181,157,243, 0,1512,1761,2584,4213,4387,5882,6738,7027,8407,
1 90394 103967 rna-gnl|WGS:JABURB|Cocit.A0009.1 0 + 90394 103967 0 17 450,91,119,191,211,241,416,109,200,350,97,219,240,210,286,197,882, 0,603,924,2440,2718,4600,5837,7150,7535,7867,8376,8552,9331,10114,11699,12126,12691,
1 111647 117022 rna-gnl|WGS:JABURB|Cocit.A0010.1 0 + 111647 117022 0 8 264,129,855,246,24,141,48,471, 0,1487,1745,3106,3458,3742,4009,4904,
FASTA:
>rna-gnl|WGS:JABURB|Cocit.A0001.1
ATGACAGCCCTCAAGCTCAAGAAGCTCCTCCTGACCGCCATCGCGGTCGCTGGGATCGTTGTCTCTGCTCTGCCTGACACCGCCTCGGCCCAGAACTGCGGGTGTGCAGCCAACC
Maybe it is because of the symbols in the transcript names?