how_are_we_stranded_here
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Error: kallisto index file not found kallisto_index
Hello, I tried to get this tool running but encounter an issue. The following are the code and error messages that I got:
Code:
check_strandedness --gtf ref/Mus_musculus.GRCm38.93.gtf --transcripts ref/Mus_musculus.GRCm38.cdna.all.fa --reads_1 reads/test_R1.fastq.gz --reads_2 reads/test_R2.fastq.gz
Output error:
Error: kallisto index file not found kallisto_index
Usage: kallisto quant [arguments] FASTQ-files
...
xxxxxxxx/kallisto_strand_test/pseudoalignments.bam does NOT exists.
Traceback (most recent call last):
File "xxxxxxxx/check_strandedness", line 8, in <module>
sys.exit(main())
File "xxxxxxxx/site-packages/how_are_we_stranded_here/check_strandedness.py", line 185, in main
result = pd.read_csv(test_folder + '/' + 'strandedness_check.txt', sep="\n", header=None)
File "xxxxxxxx/site-packages/pandas/util/_decorators.py", line 311, in wrapper
return func(*args, **kwargs)
File "xxxxxxxx/site-packages/pandas/io/parsers/readers.py", line 665, in read_csv
kwds_defaults = _refine_defaults_read(
File "xxxxxxxx/site-packages/pandas/io/parsers/readers.py", line 1538, in _refine_defaults_read
raise ValueError(
ValueError: Specified \n as separator or delimiter. This forces the python engine which does not accept a line terminator. Hence it is not allowed to use the line terminator as separator.
can you provide some suggestions for solving this issue?
The software version on my side is:
how-are-we-stranded-here 1.0.1
rseqc 4.0.0
kallisto 0.48.0
python 3.10.5
also tested for kallisto 0.44.0 and python 3.7.7, with the same issue