Cheng-Kai Shiau

Hi Minoru, The error is due to insufficient memory when running qualimap with default java setting. Please check this to increase the memory usage: https://stackoverflow.com/questions/1565388/increase-heap-size-in-java I've updated and added a...

Hi Minoru, The error message showed that when qualimap preparing files it couldn't find bgzip: ``` samfaipath] build FASTA index... [E::fai_build3] Cannot index files compressed with gzip, please use bgzip...

Hi, The error message could be due empty single cell FastQ file which opened by pysam module. I think something wrong in scanner step. Could you check your scanner log...

Hi Lily, Please try to install python 3.9. I never try python 3.7 because it's out of date. Many tools/libraries are deprecated and no longer maintained on 3.7. The first...

Hi Minoru, Thank you for your feedback. Could you provide your assigner log file ? Furthermore, could you please try to delete the whole temporary folder ("tmp" by default), and...

Hi Minoru, How many cells are you target to ? In the assigner log file, it detected 160 cells. Could you try provide "CB_log10_dist.png" under result folder ? Thanks. Regards,...

Hi Lily, How many cell are you target to ? Could you provide assigner log file and CB_log10_dist.png under result folder? Thanks. Regards, Cheng-Kai > Hi Cheng-Kai, > > Many...

Hi Luyang, Sorry for the late reply. Just get back from a week long conference. From your CB_log10_dist figure, probably you are targeting to ten thousands or twenty thousands of...

Hi Minoru, Sorry for the late reply. Just get back from a week long conference. Thank you for your update. I'm glad to help answering any question. Regards, Cheng-Kai >...

I'm closing this issue. Please feel free to open it if you have any further questions. Thanks.