Sjors Scheres

icon indicating a website link http://www2.mrc-lmb.cam.ac.uk/groups/scheres

icon company MRC Laboratory of Molecular Biology icon location Cambridge, UK icon location I'm a scientist interested in new methods for cryo-electron microscopy strudture determination of biological macromolecules

Results 6 comments of Sjors Scheres

Blush with no-align Class3D

Dari, how can one run a single execution of blush on a map? One would need to compare that with the blushed Class3D.On 8 Nov 2023 17:56, Oliver Clarke ***@***.***>...

Estimating initial noise spectra from HUGE number of particles

I suspect this is caused by some conversion issue between relion and M. Perhaps in the generation of the star files.

CTF estimation creates symlinks with absolute paths

Just want to confirm that STA behaves in the exact same way as SPA here. The PS.mrc files get generated during motioncorr and are only temporarily symlinked. Yes, deleting them...

Relion 5 tomography polishing generates a miniaturised map inside the main map

It is essential to use a bin1 reconstruction for running CTF refinement or polishing.

Relion 5.0 + AMD MI210, AMD Milan, SLURM, OpenMPI cannot allocate on GPU

Does the same happen without MPI?

Relion 5.0 + AMD MI210, AMD Milan, SLURM, OpenMPI cannot allocate on GPU

OK, then the problem has nothing to do with the openmpi. Perhaps @suyashtn can comment?