satomimitsuhashi

Thank you for the reply! psl files were not empty. I had 2.8G fasta file and got 2.6M of gmapoutput.psl and 1.6M of gmapoutput_filtered.psl. Is it normal? By the way,...

Dear Chris, I am trying to sequence cDNA from human skeletal muscle using R9.5 flow cell and 1D^2 kit. The reads were unexpectedly short in this run. There might be...

Median read length was 1349bp. I did not check the size in detail but the peak was more than 3kb on a gel. I think last can detect splicing now,...

Thank you very much! That was very helpful. I will let you know how it works. Best, Satomi > 2017/09/13 8:57、christopher-vollmers のメール: > > You are right. LAST might just...

Dear Chris, I tried this, > If you have the psl you can simply > change the name to "2D_trimmed_l_gmapoutput.psl" and rerun Mandalorian_4 > but comment out the alignment step...