how to quantify the expression of one gene in more conditions?

[feanaros]

Hi,

I have 4 experimental conditions: WT (2 reps) KO1 (2 reps) KO2 (2 reps) KO3 (2 reps)

I would like to compare the expression of WT against the 3 mutants. I want to use something similar to FindMarkers(seurat, features = "mygene"), but I want to set ident.1 = WT and ident.2 = KO1, KO2, KO3 (analyzing them together while still being able to distinguish between them). Is this possible? Additionally, is there a way to visualize the results in a heatmap with Z-scores?

Thank you!