All cells have the same value of blacklist_ratio.

[GaF123]

Hi, when I run:

compute nucleosome signal score per cell

pbmc <- NucleosomeSignal(object = pbmc)

compute TSS enrichment score per cell

pbmc <- TSSEnrichment(object = pbmc, fast = FALSE)

add blacklist ratio and fraction of reads in peaks

pbmc$pct_reads_in_peaks <- pbmc$peak_region_fragments / pbmc$passed_filters * 100 pbmc$blacklist_ratio <- pbmc$blacklist_region_fragments / pbmc$peak_region_fragments

It showed: Warning message: In SingleExIPlot(type = type, data = data[, x, drop = FALSE], idents = idents, : All cells have the same value of blacklist_ratio.

summary(combined$blacklist_region_fragments) Min. 1st Qu. Median Mean 3rd Qu. Max. 0 0 0 0 0 0 table(combined$blacklist_region_fragments)

0 

35953

Please help, thank you!