Do we need to split merged object back to its individual objects in order to re-run SCTransform after removing some clusters?

[denvercal1234GitHub]

Hi there,

It was advised to re-run SCTransform to get a new vector of variable genes after removing cells from the object.

Nevertheless, given that SCTransform is recommended to be run on individual objects (and not on the merged object) in order to learn dataset-specific variation, after I removed cells from the merged object, does this mean I will need to split the merged object back to individual objects so that I can run SCTransform? Then I will remerged these new individual datasets?

Thank you for your advice.