SCTransform taking hours with very large memory demand

[liqianma7]

I have a very large dataset (Seurat object ~8gb) and sctransform takes quite long time and eventually failed due to memory issue (RAM=80gb). In this case, would you recommend doing sctransform only on variable features? Will this affect DEG analysis downstream?

Thank you very much for your help!

[ChristophH]

What are the dimensions of your input matrix? What are the exact commands you use? It's hard to pinpoint the issue without that information. If you are using Seurat::SCTransform you might want to set conserve.memory = TRUE