SCTransform Normalize Sequencing Depth

Open ShaowenJ opened this issue 5 years ago • 1 comments

Hi, I just have a quick question, How does SCTransform Normalize Sequencing Depth across all cells, do you need to specify the argument vars.to.regress = "nCount_RNA" in the SCTransform function? Or it automatically would do that?

Thanks!

Mar 19 '20 19:03 ShaowenJ

And one more question if you don't mind, What's the difference between "ScaleData(obj, vars.to.regress = "nCount_RNA")

Thanks!

Mar 19 '20 19:03 ShaowenJ