azimuth
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TPM count matrix for Azimuth annotation
Dear Authors,
I am analyzing Smart-seq2 single cell RNA-seq data, which is a full length RNA-seq data. TPM removes transcript length difference effects from the data. So, I wonder if I can use the TPM count matrix for Azimuth annotation or I have to use the raw count matrix data since Azimuth performs normalization by itself.
And I wonder If there is a way around of Azimuth's normalization to avoid duplicating normalization in the case I use TPM.
Thank you so much for your help. Luke