Y24-194 - LCM WGS/EM-Seq - Pipeline configuration and integration test

[andrewsparkes]

User story As Scott and Sara we would like tracking developed for the new LCM WGS/EM-Seq pipeline. A process map and associated documentation contain the details required.

Who are the primary contacts for this story Scott G (R&D) Sara W (Lab ops)

Who is the nominated tester for UAT TBC

Acceptance criteria Sub-stories have been created that should cover this story. The following acceptance criteria are for checking everything is joined up and tested:

• [ ] Integration Suite test for whole pipeline, including the same samples going down both the EM-Seq and WGS branches.

Dependencies This story is divided into two stories for EMSeq and WGS branches and a common initial part up to the Adp Lig plate. Transitive dependencies have been added here as well to find them easier.

• [x] Y24-271 - Configuration and integration test for the start of LCM Triomics Pipeline
• [x] Y24-266 - LCM Triomics Pipeline configuration and integration test for EMSeq branch
• [ ] Y24-267 - LCM Triomics Pipeline configuration and integration test for WGS branch
• [ ] Y24-268 - Create a presenter for automated submission for WGS branch on Adp Lig plate

References This story has a non-blocking relationship with:

• Y24-196 - LCM WGS/EM-Seq - Research - Test pipeline configuration This is the research story about the branch point.
• LCM WGS/EM-Seq Bed verifications This is the following story for bed verifications.

Additional context See the following documents for detailed information (e.g. steps and plate purposes): LucidChart Process Diagram Overview document CLCM Multiomics document

Mostly this story is standard SS and Limber configuration. These are the tricky bits I can see (also see docs above):

1. Submissions - Initial submission is on the Lysate plate at the start, for DNA prep (RNA branch may be added later). Then part way along this brnaches into WGS and EM paths. Both branches will always happen, in parallel. You cannot use one submission all the way through because of this later branch. But as both will always happen for all samples then you don't want to have to wait on the SSR to manually create submissions at the branch point. Possibly create an automatic submission to branch off the EM path? Have to try out some options and see what works best.
2. There are several places where they have annotated bed verifications that want to check multiple plates in advance. We can't do bed verifications that involve transfers that go A->B->C, but we should be able to do verifying ones that check plates are in specific positions. But only if LIMS already knows about the barcodes of those plates, so they'd have to be set up in advance. Includes crossing the branching point.
3. They would like to print out labels in advance for these steps that include the branching. I'm not sure we can do that.

The research story for the branching point is Y24-196 - LCM WGS/EM-Seq - Research - Test pipeline configuration