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Why the busco scores for the annotated example arabidopsis thaliana genome proteins are very low ?

Open bioinformaticspcj opened this issue 2 years ago • 3 comments

Dear the authors,

Thanks a lot for your valuable and user-friendly software. I have tried the finder programe to annotate the example arabidopsis thaliana genome, but found the proteins extrated from FINDER_BRAKER_PROT.gtf file only achieved 54% BUSCO scores of embryophyta_odb10. It is very low compared with the genome itself BUSCO scores 99.3% with the same database and means near half of coding genes were not annotated. I do not know why. The command I used to run finder is as fellows: finder -no_cleanup -mf Arabidopsis_thaliana_metadata.csv -n 30 -gdir_star $PWD/star_index_without_transcriptome -out_dir $PWD/FINDER_test_ARATH -g $PWD/Arabidopsis_thaliana.TAIR10.dna_sm.toplevel.fa -p $PWD/uniprot_ARATH.fasta -gdir_olego olego_index -preserve 1> $PWD/FINDER_test_ARATH.output 2> $PWD/FINDER_test_ARATH.error

Could you give me some advice to improve the performence of the annotation?

Thanks again for your reading. Looking forward to your timely respons. Best, Bob

bioinformaticspcj avatar Dec 01 '21 13:12 bioinformaticspcj