Minipolish icon indicating copy to clipboard operation
Minipolish copied to clipboard

Added functionality for PacBio CCS/HiFi reads

Open jelber2 opened this issue 5 years ago • 3 comments

Thanks for developing this great tool! I just wanted to add the functionality to be able to use PacBio CCS/HiFi reads. I haven't really tested if this is an improvement or not.

jelber2 avatar Feb 26 '20 12:02 jelber2

Hmmm. I didn't notice a difference in the assembly results between

 minipolish --pacbio clr --threads 75 $read_overlaps $unpolished_assembly

and

minipolish --pacbio ccs --threads 75 $read_overlaps $unpolished_assembly

So, I wonder if I correctly implemented the --pacbio option in the python code.

jelber2 avatar Mar 02 '20 07:03 jelber2

Okay, I think I fixed this. So now --pacbio clr --pacbio ccs or --pacbio no options work. Also changed the README.md and simple running script.

jelber2 avatar Apr 05 '20 08:04 jelber2

I also added the functionality of using winnowmap or minimap2 for aligning long reads to the miniasm and polished assemblies. The commits have not been exhaustively tested for errors/bugs.

jelber2 avatar Apr 07 '20 07:04 jelber2