SnapATAC icon indicating copy to clipboard operation
SnapATAC copied to clipboard

Published 20 hours ago • verified publisher icon r3fang

Possible bug in snaptools dex-fastq, on snATAC dataseq generated with latest 10X snATAC V1.1 chemistry

Open Qi-Yang-Bioinfo opened this issue 4 years ago • 0 comments

I was running SnapATAC on a dataset generated with latest 10X snATAC V1.1 chemistry, from scratch(Not on cell ranger results). It seems that snaptools dex-fastq is not aware of the change of barcode position, thus grabbed the wrong part and treated as barcode.

I was running SnapATAC R and No common barcode exist between snapobject@barcodes and 10X_singlecell_file@barcodes, wired barcode sequence shown in snapobject@barcodes like AAAAAAAAAAAAAAAA(shouldn't exist) made me believe this is a bug in snaptools dex-fastq

Qi-Yang-Bioinfo avatar Dec 25 '20 03:12 Qi-Yang-Bioinfo