Applying SL, TMM, and IRS on log2 data or raw data

[lisiarend]

Hey, I have a question regarding the three normalization methods that you applied. Actually it makes a difference whether to apply for example SL on raw data and log2 the data for visualization, or to apply SL on already log2 transformed data. The same results for TMM and IRS.

What is best practice for this? And why would you (as in your markdown) use these methods on raw data?

Best, Lis