Wild Variability in doublet scores

[markddesimone]

Hi, thank you for the excellent tool. I was looking at the variability in scores between different runs on the same data and was very surprised to see barcodes called as doublets with very high probability in one run and almost zero probability in another. here's a scatter plot of one run's scores vs another: I was expecting variability of course, but not so dramatic. I'm not sure what to do with these results. For example I can run scDblFinder say 10 times and threshold on a high level of agreement on called doublets; or I could take the union of all called doublets on the 10 runs; or something in between. I'm interested in your opinion as to the better approach and the reasoning behind that.

Thank you