CRISPRme
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UnicodeDecodeError: 'utf-8' codec can't decode byte 0x90 in position 48:cluster.dict.py
I have faced the problems
hg38+hg38_1000G_test_20bp-NGG-SpCas9.txt_guides.txt_gencode_encode.hg38.bed_6_2_2_chrX_KI270881v1_alt.total.cluster.txt.tmp_sort.txt file have specific symbols
<90> <E0> in third columns. And I had faced with error blows,I want to know if this problem can be solved,any ideas about how to solve this problem would be very grateful.
run command line
crisprme.py complete-search --genome Genomes/hg38 --vcf list_vcf.txt/ --guide sg1617.txt --pam PAMs/20bp-NGG-SpCas9.txt --annotation Annotations/gencode_encode.hg38.bed --samplesID list_samplesID.txt --gene_annotation Gencode/gencode.protein_coding.bed --bMax 2 --mm 6 --bDNA 2 --bRNA 2 --merge 3 --output sg1617.6.2.2_new --thread 58
8. Annotation
I download the docker crispr using command line:
docker pull pinellolab/crisprme
file format
the third column have specific symbols,<90><E0>
Any help tracing this down would be helpful, I am looking forward to running the tool on a real sequence!
Let me know if any other information would be helpful... khl