CRISPResso2

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CRISPResso2_Pooled

2

Hello, I use the CRISPRessoPooled command to analyze several cleavage sites in the bacterial genome. Previously, it was ok, but the Pooled version stopped working at some point. Now I...

Nikita-Sharaev

CRISPRessoWGS failed

5

Hi, I tried to analyse an amplicon >1kb long with single end reads. I made my amplicon into an indexed reference .fa file by bowtie2 to which I aligned my...

Theos1231008

barcodes

1

Does CRISPResso2 demultiplex if the barcode sequences are supplied?

weiss480

quantification window ERROR and invalid characters ERROR when using prime-edit

2

**Describe the bug** I encounter tow type of errors while using CRISPResso2. The ERRORs are **quantification window ERROR** and **invalid characters ERROR** I upload all the fastq.gz file [here](https://mahhlab.org/downloads/) ##...

Masterchiefm

match dsODN sequences

2

**Is your feature request related to a problem? Please describe.** Current dsODN sequence matching doesn't allow mismatches `df_alleles["Aligned_Sequence"].str.find(args.dsODN) > 0` **Describe the solution you'd like** we could just do a...

YichaoOU

Batch CRISPRessoPooled

1

I really appreciate the Batch function, but I would like to have a feature for multiple (batch) of CRISPRessoPooled samples. I have to currently make a custom script to make...

jsacco1

CRISPResso2 cannot detect HDR

5

I have tried to use CRISPResso version 2.0.31 to process my NGS HDR data. For some reason, I input the HDR sequence as the format of the amplicon but in...

lvelve0901

Crispresso without adapter trimming

3

I have recently found out some of my samples have adapters in the fastq files and I have not used the "trim adapter" command. The crispresso results are as expected...

Sahus2

ERROR: Cannot set a frame with no defined index and a value that cannot be converted to a Series

2

Dear sir, When I tried to use : "CRISPRessoWGS -b 1K/PCR-KL20211111-ALAS1-1_S52_L002.sorted.bam.bam -f guide.txt -r ALAS1_ngs1k.fasta" It shows massage like ~~~CRISPRessoWGS~~~ -Analysis of CRISPR/Cas9 outcomes from WGS data- _ _ '...

deb0612

doesn't seem to menage inversion

1

After the editing with two guides, I got two alleles, one long like the WT and a shorter one. For crispresso2 one has the expected deletion between the guides and...

cangelon