Hi, i even with the new version got this error "ZeroDivisionError: float division by zero"
this is the code i run:
python ncm.py -B -l M1M2.txt -bed SNP_GRCh38_hg38_wChr.bed -O M1M2_results
WARNNING : NCM_HOME is not defined yet. Therefore, program will try to search ncm.conf file from the current directory
1.6
Note: none of --samples-file, --ploidy or --ploidy-file given, assuming all sites are diploid
Set max per-file depth to 8000
/home/seisbio/mambaforge/envs/ngscheckmate-env/bin/samtools mpileup -uf GCA_000001405.29_GRCh38.p14_genomic.fna -l SNP_GRCh38_hg38_wChr.bed M2_dedup_filtrado_sorted.bam | /home/seisbio/mambaforge/envs/ngscheckmate-env/bin/bcftools call -c > M1M2_results/M2_dedup_filtrado_sorted.vcf
Note: none of --samples-file, --ploidy or --ploidy-file given, assuming all sites are diploid
[mpileup] 1 samples in 1 input files
Set max per-file depth to 8000
Generate Data Set from M1M2_results
using this bed file : SNP_GRCh38_hg38_wChr.bed
Traceback (most recent call last):
File "ncm.py", line 1453, in
createDataSetFromDir(base_dir,bedFile)
File "ncm.py", line 222, in createDataSetFromDir
real_depth[file] = depth[file] / float(real_count[file])
ZeroDivisionError: float division by zero
PLEASE HELP!!!
parklab
