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Issue obtaining unaligned reads

Open DanLesman opened this issue 1 year ago • 2 comments

I am interested in separating out my reads that are aligning and not aligning after using kb count. I modified kallisto so that the -n argument is passed. I then run bustools text -f -o bus.txt output.bus to try and see the line numbers of the aligned reads so I can determine which reads are aligning, but every value in the final column is 0. Not sure if I'm doing something wrong, if there is a bug, or if there is a better way of obtaining the unaligned reads.

Thank you!

DanLesman avatar Apr 20 '24 23:04 DanLesman

Are you using "kb count"? Try specifying --num instead of -n.

Yenaled avatar Apr 21 '24 11:04 Yenaled

This works, thank you!

DanLesman avatar Apr 22 '24 12:04 DanLesman

This issue is stale because it has been open 30 days with no activity. Remove stale label or comment or this will be closed in 5 days

github-actions[bot] avatar May 23 '24 00:05 github-actions[bot]