kallisto
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kallisto velocity pipeline bug ?!
Hi, In the velocity pipeline you have the following:
cd ./output/neuron10k_velocity
bustools correct -w ../../data/whitelist_v3.txt -p output.bus | \
bustools sort -o output.correct.sort.bus -t4 -
bustools capture -s -x -o spliced.bus -c ./introns_tx_to_capture.txt -e matrix.ec -t transcripts.txt output.correct.sort.bus
bustools capture -s -x -o unspliced.bus -c ./cDNA_tx_to_capture.txt -e matrix.ec -t transcripts.txt output.correct.sort.bus
It is not that for spliced you need the cDNA_tx_to_capture.txt
, and for the unspliced, you need the introns_tx_to_capture.txt
?
Thanks, HM