loci.formats.FormatException while converting series to tiff format

[de9821]

my current version bftools is below

Version: 7.3.1 Build date: 11 July 2024 VCS revision: 41e12f630ad7349caa0293cba23948c17d22732b

I am getting below error while converting vsi file series into tiff

../bftools/bfconvert -no-upgrade -bigtiff -series 1 -pyramid-scale 1 -noflat ./DATA_022/DATA_022.vsi ./DATA_022_1.tiff ./DATA_022/DATA_022.vsi CellSensReader initializing ./DATA_022/DATA_022.vsi [CellSens VSI] -> ./DATA_022_1.tiff [Tagged Image File Format] Tile size = 512 x 512 Converted 1/1 planes (100%) Tile size = 512 x 512 Exception in thread "main" loci.formats.FormatException: (w:512 + x:3584) must be <= 4034 at loci.formats.FormatWriter.checkParams(FormatWriter.java:469) at loci.formats.out.TiffWriter.saveBytes(TiffWriter.java:223) at loci.formats.out.TiffWriter.saveBytes(TiffWriter.java:469) at loci.formats.ImageWriter.saveBytes(ImageWriter.java:260) at loci.formats.tools.ImageConverter.convertTilePlane(ImageConverter.java:1076) at loci.formats.tools.ImageConverter.convertPlane(ImageConverter.java:908) at loci.formats.tools.ImageConverter.testConvert(ImageConverter.java:841) at loci.formats.tools.ImageConverter.main(ImageConverter.java:1318)

Series description is below

Image count = 1 RGB = true (3) Interleaved = true Indexed = false (false color) Width = 4034 Height = 4710 SizeZ = 1 SizeT = 1 SizeC = 3 (effectively 1) Tile size = 512 x 512 Thumbnail size = 109 x 128 Endianness = motorola (big) Dimension order = XYCZT (uncertain) Pixel type = uint8 Valid bits per pixel = 8 Metadata complete = true Thumbnail series = false

Plane #0 <=> Z 0, C 0, T 0

I referred documentation and tried various options but still getting error.

Also tried older version of 6.3.1 as it was suggested in old discussion in forum but no luck.

please suggest if you faced similar issue and have any solution

[dgault]

Hi @de9821, thank you for reporting the issue. If you leave out the -pyramid-scale 1 option, do you still see the same error?

[de9821]

Hi @dgault thanks for the response.

I see similar error with out -pyramid-scale 1 option.

../bftools/bfconvert -no-upgrade -bigtiff -series 1 -noflat ./DATA_022/DATA_022.vsi ./DATA_022_1.tiff ./DATA_022/DATA_022.vsi CellSensReader initializing ./DATA_022/DATA_022.vsi [CellSens VSI] -> ./DATA_022_1.tiff [Tagged Image File Format] Tile size = 512 x 512 Converted 1/1 planes (100%) Tile size = 512 x 512 Exception in thread "main" loci.formats.FormatException: (w:512 + x:3584) must be <= 4034 at loci.formats.FormatWriter.checkParams(FormatWriter.java:469) at loci.formats.out.TiffWriter.saveBytes(TiffWriter.java:223) at loci.formats.out.TiffWriter.saveBytes(TiffWriter.java:469) at loci.formats.ImageWriter.saveBytes(ImageWriter.java:260) at loci.formats.tools.ImageConverter.convertTilePlane(ImageConverter.java:1076) at loci.formats.tools.ImageConverter.convertPlane(ImageConverter.java:908) at loci.formats.tools.ImageConverter.testConvert(ImageConverter.java:841) at loci.formats.tools.ImageConverter.main(ImageConverter.java:1318)

[dgault]

Thanks @de9821 for confirming. Would you be able to share a sample file showing the error that we can test? If you need a suitable upload location then you can upload files to the Bio-Formats Zenodo community.