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MetaMorph: Incorrect parsing of laser metadata

Open dgault opened this issue 4 years ago • 1 comments

This issue was first raised on imagesc thread https://forum.image.sc/t/metamorph-stack-issue-with-ome-metadata-bioformats-and-omero/48416 and a sample dataset was provided which reproduces the issues reported with Bio-Formats 6.6.0

There are 3 separate issues with the dataset which are as below (taken from the imagesc thread):

  • The folder 60x_2well_all100_G50ms_C100ms contain data that was acquired by setting the exposure time for the 488nm laser to 50ms and the exposure time for the 640nm laser to 100ms. However, from the OME metadata, it appears that all channels have the same exposure time (50ms).
  • Also, it incorrectly assigns the emission wavelength to the laser wavelength (the same 525nm for the two lasers, sdcGFP and sdcCy5.
  • Also, in the OME metadata, DeltaT is set to 0 starting from the 6th timepoint, although the user have acquired images every 20s.

801e943a565f6d30041190d1dffeff008a2b047c

dgault avatar Feb 11 '21 13:02 dgault

This issue has been mentioned on Image.sc Forum. There might be relevant details there:

https://forum.image.sc/t/metamorph-stack-issue-with-ome-metadata-bioformats-and-omero/48416/8

imagesc-bot avatar Feb 11 '21 13:02 imagesc-bot