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VSI: incorrect exposure times

Open dgault opened this issue 4 years ago • 4 comments

This issue was raised in forum thread https://forum.image.sc/t/bio-formats-exposure-time-incorrect-when-importing-vsi-files/46080

In the OME-XML the Exposure Time is the same for each plane. However the correct values can be found in the original metadata tags as shown in the images attached in the imagesc thread.

dgault avatar Dec 03 '20 10:12 dgault

This issue has been mentioned on Image.sc Forum. There might be relevant details there:

https://forum.image.sc/t/bio-formats-exposure-time-incorrect-when-importing-vsi-files/46080/2

imagesc-bot avatar Dec 03 '20 11:12 imagesc-bot

https://trello.com/c/v47N2hGC/382-vsi-incorrect-metadata-posz-and-exp-time might be related.

melissalinkert avatar Dec 03 '20 16:12 melissalinkert

A sample file showing the issue has been received in QA-29751 and the issue was tested and reproduced with BF 6.5.1

In the OME-XML the exposure time remains the same across planes:

<Plane ExposureTime="1.0" ExposureTimeUnit="s" PositionX="-82449.09870917775" PositionXUnit="µm" PositionY="-68454.64727965342" PositionYUnit="µm" TheC="0" TheT="0" TheZ="0"/>
<Plane ExposureTime="1.0" ExposureTimeUnit="s" PositionX="-82449.09870917775" PositionXUnit="µm" PositionY="-68454.64727965342" PositionYUnit="µm" TheC="1" TheT="0" TheZ="0"/>
<Plane ExposureTime="1.0" ExposureTimeUnit="s" PositionX="-82449.09870917775" PositionXUnit="µm" PositionY="-68454.64727965342" PositionYUnit="µm" TheC="2" TheT="0" TheZ="0"/>

In the original metadata the correct values are present:

Microscope Exposure time (microseconds) #1	150000
Microscope Exposure time (microseconds) #2	250000
Microscope Exposure time (microseconds) #3	1000000

dgault avatar Dec 04 '20 11:12 dgault

This issue has been mentioned on Image.sc Forum. There might be relevant details there:

https://forum.image.sc/t/estimating-stain-vectors-no-brightfield-rgb-image-selected-error-message-keeps-popping-up-and-cannot-resolve/66246/5

imagesc-bot avatar Apr 29 '22 20:04 imagesc-bot