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Genome mapping and spliced alignment of cDNA or amino acid sequences

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Hi Gotoh, Recently, I'm trying to replace genewise with your spaln. I found spaln could get more expected gene structure as query protein. It seems great! To apply in non-model...

First, some results would be converted to 0-based start in gff3 output. I think it's the fault with the deletion of the first base. For example: ![image](https://user-images.githubusercontent.com/9262911/57844583-f0661380-7802-11e9-9e13-3ca9e2d61acb.png) the gff3 output:...

Dear developers, I got the results from spaln aligning CDS to the reference genome and I want to perform some filters based on the 6th column of the gff3 output...

Hello, I'm using a reference cDNA set of a gene family against a highly fragmented draft assembly of a closely related species and I'm getting hits on multiple contigs. For...

Hi, When I ran the new version Spaln 2.3.1 with command: spaln -dGenome test.fa It reported "Can't open query !". Any idea why? Thanks!

Hi When I aligned the protein seq to my genome, I got a GFF file. But when I used this GFF file for Maker pipeline. I got an error. The...

Unable to run newest version of spaln using existing database and input: IldModel.txt not found! Confirm whether ALN_TAB is correctly set please advise.

While trying to create a bioconda recipe for spaln, I ran into an issue concerning long pathnames generated during the conda build process that cause an error during `make install`:...

Hi, We have realized that after executing the command pasted below, the output gff3 contains some cds with 4th field 1 base greater than the 5th field. This issue generates...

Hello, I found spaln crashing on this command, when -D_FORTIFY_SOURCE=3 is used during build 345s autopkgtest [20:14:46]: test run-unit-test: [----------------------- 346s Gd:187913 No:71174 My:3057 MS:157 Mb:188 Tw:2331733 Tl:33850852 71.68 1.17...