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some question about using scisorseqr

Open yuanwsy opened this issue 2 months ago • 3 comments

Hi,Thanks for developing such a good tool! When I am using the getbarcodes step ,I use the following command: GetBarcodes(fqFolder = fastqFolder, BCClustAssignFile = bc_clust, outputFolder="/data/R02/yuanwsy/scisorseqr/scisorseqresult/", chemistry = "v3", concatenate = TRUE, filterReads = FALSE)

and the bc_clust looks like this ,the same as the example

V1 V2
TCGCAGGGTGTCATTG Macrophage
CTTGATTGTGTTCGTA Pericyte
NA NA
TACATTCAGCCAACCC B cell
ATTCGTTGTGCCGTAC iOSN
TAGGTACAGAGCCGAT B cell
CATCCGTCAAGTCATC HBC
CAGCGTGCAAGTACCT Neutrophil

but after I ran the command line ,It came to an error like this: cat: ./OutputRaw//DeconvBC*.csv: No such file or directory

I can not find the the information of DeconvBC*.csv in the vignette ,do you know how to solve it? Thanks!

yuanwsy avatar Apr 18 '24 14:04 yuanwsy