scisorseqr
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some question about using scisorseqr
Hi,Thanks for developing such a good tool! When I am using the getbarcodes step ,I use the following command: GetBarcodes(fqFolder = fastqFolder, BCClustAssignFile = bc_clust, outputFolder="/data/R02/yuanwsy/scisorseqr/scisorseqresult/", chemistry = "v3", concatenate = TRUE, filterReads = FALSE)
and the bc_clust looks like this ,the same as the example
V1 | V2 |
---|---|
TCGCAGGGTGTCATTG | Macrophage |
CTTGATTGTGTTCGTA | Pericyte |
NA | NA |
TACATTCAGCCAACCC | B cell |
ATTCGTTGTGCCGTAC | iOSN |
TAGGTACAGAGCCGAT | B cell |
CATCCGTCAAGTCATC | HBC |
CAGCGTGCAAGTACCT | Neutrophil |
but after I ran the command line ,It came to an error like this: cat: ./OutputRaw//DeconvBC*.csv: No such file or directory
I can not find the the information of DeconvBC*.csv in the vignette ,do you know how to solve it? Thanks!