image registration

[rojinsafavi]

I have 4 immunofluorescence images from 4 adjacent slides (in tiff), which should cover ~2-cell in z-stack. I need to align those slides (I think it is called image registration).

Ideally, I need to make a pipeline that aligns these four images into a single one, and outputting the "moving" and "rotation" matrices of each image.

Is that a possible thing with your package?

Thank you very much!