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PLOT_CONSENSUS_PEAKS worflow does not produce the upset plot
Description of the bug
Hello,
The workflow NFCORE_CUTANDRUN:CUTANDRUN:PEAK_QC:PLOT_CONSENSUS_PEAKS
is run with 0 exit code, however, while the folder ${params.outdir}/04_reporting/consensus_upset_plots
is created, the upset plots are not produced.
Here is the working directory of the workflow:
{workDir}/work/f5/f4[...]/
├── .command.begin
├── .command.err
├── .command.log
├── .command.out
├── .command.run
├── .command.sh
├── .command.trace
├── .exitcode
├── {GROUP 1}.macs2.consensus.peak_counts.bed -> {workDir}/work/f1/2eea337cf57ff46f6b3b77d9fb8265/{GROUP 1}.macs2.consensus.peak_counts.bed
├── {GROUP 2}.macs2.consensus.peak_counts.bed -> {workDir}/work/ed/19a8a14d34c607dcdaedd4153f8176/{GROUP 2}.macs2.consensus.peak_counts.bed
└── versions.yml
And here is .command.sh
:
#!/bin/bash -euo pipefail
plot_consensus_peaks.py \
--peaks "*.peaks.bed" \
--outpath .
cat <<-END_VERSIONS > versions.yml
"NFCORE_CUTANDRUN:CUTANDRUN:PEAK_QC:PLOT_CONSENSUS_PEAKS":
python: $(python --version | grep -E -o "([0-9]{1,}\.)+[0-9]{1,}")
numpy: $(python -c 'import numpy; print(numpy.__version__)')
pandas: $(python -c 'import pandas; print(pandas.__version__)')
upsetplot: $(python -c 'import upsetplot; print(upsetplot.__version__)')
END_VERSIONS
After modifying the script:
section in modules/local/python/plot_consensus_peaks.nf
to:
plot_consensus_peaks.py \
--peaks "*.peak_counts.bed" \
--outpath .
cat <<-END_VERSIONS > versions.yml
"NFCORE_CUTANDRUN:CUTANDRUN:PEAK_QC:PLOT_CONSENSUS_PEAKS":
python: $(python --version | grep -E -o "([0-9]{1,}\.)+[0-9]{1,}")
numpy: $(python -c 'import numpy; print(numpy.__version__)')
pandas: $(python -c 'import pandas; print(pandas.__version__)')
upsetplot: $(python -c 'import upsetplot; print(upsetplot.__version__)')
END_VERSIONS
it works as expected.
Command used and terminal output
nextflow run nf-core/cutandrun -profile singularity -params-file params.yaml --input samplesheet.csv
params.yaml
input: "./samplesheet.csv"
outdir: "./results"
genome: "GRCh38"
peakcaller: "MACS2,SEACR"
dt_calc_all_matrix: true
use_control: false
skip_igv: true
### Relevant files
_No response_
### System information
Nextflow: 24.04.3.5916
Hardware: HPC
Executor: PBS
Container engine: Singularity
OS: Rocky Linux 8.10 kernel 4.18.0
Architecture: x86-64
nf-core/cutandrun: tested with both 3.2.2 and master