nextclade
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Alternative Splicing
I was thinking of making Nextclade datasets for the human adenoviruses, starting with hAdV-F (NCBI accession: NC_001454.1), but I ran into a snag almost right away. The expression of certain genes uses alternative splicing, but Nextclade has a hard time reconciling discontiguous coding segments that contribute to the same polypeptide, especially when an intron interrupts a codon.
For example, the E1A gene has an intron that splits the coding region in the middle of a codon.