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nextgenusfs
Error because funannotate is not able to find the "/predict_misc/protein_alignments.gff3" file.
Hi funannotate team,
First, congratulations on the development of funannotate, is a great annotation tool for fungal genomes, I am having great fun using it!
I am trying to annotate some yeast genome sequences, but I am getting into trouble with the latest funannotate version, 3.8.13.
Although the workstation where I am running the code is a bit old, I think I am not commiting any serious mistake. The command I am using is the following:
FUNANNOTATE COMMAND USED TO ANNOTATE THE GENOME SEQUENCE:
funannotate predict -i ../0_B_Repeatmask_sequences/genome_3.fasta -s Y1_[candida]ascalaphidarum_1 -o Y1_[candida]ascalaphidarum_1.fasta --protein_evidence /home/workstation/Genomas_Pipeline_Annotation_FROM_INITIAL_GENOME_ANNOTATION_PIPELINE_SACCHAROMYCOTINA/0_0_DB_FINAL/reference_proteins.fasta $FUNANNOTATE_DB/uniprot_sprot.fasta --force --cpus 20
I have "genome_3.fasta" file at the correct place, already masked, I am using only protein evidence as annotation source, and this is based on two files, on a "reference_proteins.fasta" that I have compiled and the standard "uniprot_sprot.fasta" that funannotate uses by default.
The files and directories inside the "predict_misc" directory are
du --summarize --human-readable *
1,7M ab_initio_parameters 0 assembly-gaps.bed 11M genome.softmasked.fa 256M proteins.combined.fa 0 repeatmasker.bed
I'm guessing that the error originates from funannotate not being able to find the "/predict_misc/protein_alignments.gff3" file.
However, there is also a warning that "0.00% repeats masked", that is weird because I open the "genome_3.fasta" file and it is masked!
Bellow this message, I leave the output of the terminal and of the log files produced by funannotate, hope the funannotate team can get me some insight into this error and I will be able to overcome this problem...
Best regards,
Paulo Dias
ERROR:
[Sep 28 02:53 PM]: OS: Ubuntu 22.04, 20 cores, ~ 66 GB RAM. Python: 3.8.13
[Sep 28 02:53 PM]: Running funannotate v1.8.13
[Sep 28 02:53 PM]: Skipping CodingQuarry as no --rna_bam passed
[Sep 28 02:53 PM]: Parsed training data, run ab-initio gene predictors as follows:
Program Training-Method
augustus busco
glimmerhmm busco
snap busco
[Sep 28 02:53 PM]: Loading genome assembly and parsing soft-masked repetitive sequences
error: [Errno 2] No such file or directory: 'Y1_[candida]ascalaphidarum_1.bed/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr5.bed' run(('Y1_[candida]ascalaphidarum_1.fasta/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr5.fasta', <ValueProxy object, typeid 'Value' at 0x7f9f4fe0b760>, <AcquirerProxy object, typeid 'Lock' at 0x7f9f4fe0b790>), **{})
error: [Errno 2] No such file or directory: 'Y1_[candida]ascalaphidarum_1.bed/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr4.bed' run(('Y1_[candida]ascalaphidarum_1.fasta/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr4.fasta', <ValueProxy object, typeid 'Value' at 0x7f9f4fe0b670>, <AcquirerProxy object, typeid 'Lock' at 0x7f9f4fe0b6a0>), **{})
error: [Errno 2] No such file or directory: 'Y1_[candida]ascalaphidarum_1.bed/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr20.bed' run(('Y1_[candida]ascalaphidarum_1.fasta/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr20.fasta', <ValueProxy object, typeid 'Value' at 0x7f9f4fe145b0>, <AcquirerProxy object, typeid 'Lock' at 0x7f9f4fe145e0>), **{})
error: [Errno 2] No such file or directory: 'Y1_[candida]ascalaphidarum_1.bed/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr19.bed' run(('Y1_[candida]ascalaphidarum_1.fasta/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr19.fasta', <ValueProxy object, typeid 'Value' at 0x7f9f4fe144c0>, <AcquirerProxy object, typeid 'Lock' at 0x7f9f4fe144f0>), **{})
error: [Errno 2] No such file or directory: 'Y1_[candida]ascalaphidarum_1.bed/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr18.bed' run(('Y1_[candida]ascalaphidarum_1.fasta/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr18.fasta', <ValueProxy object, typeid 'Value' at 0x7f9f4fe143d0>, <AcquirerProxy object, typeid 'Lock' at 0x7f9f4fe14400>), **{})
error: [Errno 2] No such file or directory: 'Y1_[candida]ascalaphidarum_1.bed/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr17.bed' run(('Y1_[candida]ascalaphidarum_1.fasta/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr17.fasta', <ValueProxy object, typeid 'Value' at 0x7f9f4fe142e0>, <AcquirerProxy object, typeid 'Lock' at 0x7f9f4fe14310>), **{})
error: [Errno 2] No such file or directory: 'Y1_[candida]ascalaphidarum_1.bed/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr16.bed' run(('Y1_[candida]ascalaphidarum_1.fasta/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr16.fasta', <ValueProxy object, typeid 'Value' at 0x7f9f4fe141f0>, <AcquirerProxy object, typeid 'Lock' at 0x7f9f4fe14220>), **{})
error: [Errno 2] No such file or directory: 'Y1_[candida]ascalaphidarum_1.bed/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr15.bed' run(('Y1_[candida]ascalaphidarum_1.fasta/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr15.fasta', <ValueProxy object, typeid 'Value' at 0x7f9f4fe15100>, <AcquirerProxy object, typeid 'Lock' at 0x7f9f4fe15130>), **{})
error: [Errno 2] No such file or directory: 'Y1_[candida]ascalaphidarum_1.bed/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr2.bed' run(('Y1_[candida]ascalaphidarum_1.fasta/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr2.fasta', <ValueProxy object, typeid 'Value' at 0x7f9f4fe0b490>, <AcquirerProxy object, typeid 'Lock' at 0x7f9f4fe0b4c0>), **{})
error: [Errno 2] No such file or directory: 'Y1_[candida]ascalaphidarum_1.bed/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr14.bed' run(('Y1_[candida]ascalaphidarum_1.fasta/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr14.fasta', <ValueProxy object, typeid 'Value' at 0x7f9f4fe0bfd0>, <AcquirerProxy object, typeid 'Lock' at 0x7f9f4fe16040>), **{})
error: [Errno 2] No such file or directory: 'Y1_[candida]ascalaphidarum_1.bed/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr13.bed' run(('Y1_[candida]ascalaphidarum_1.fasta/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr13.fasta', <ValueProxy object, typeid 'Value' at 0x7f9f4fe0bee0>, <AcquirerProxy object, typeid 'Lock' at 0x7f9f4fe0bf10>), **{})
error: [Errno 2] No such file or directory: 'Y1_[candida]ascalaphidarum_1.bed/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr11.bed' run(('Y1_[candida]ascalaphidarum_1.fasta/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr11.fasta', <ValueProxy object, typeid 'Value' at 0x7f9f4fe0bd00>, <AcquirerProxy object, typeid 'Lock' at 0x7f9f4fe0bd30>), **{})
error: [Errno 2] No such file or directory: 'Y1_[candida]ascalaphidarum_1.bed/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr3.bed' run(('Y1_[candida]ascalaphidarum_1.fasta/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr3.fasta', <ValueProxy object, typeid 'Value' at 0x7f9f4fe0b580>, <AcquirerProxy object, typeid 'Lock' at 0x7f9f4fe0b5b0>), **{})
error: [Errno 2] No such file or directory: 'Y1_[candida]ascalaphidarum_1.bed/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr25.bed' run(('Y1_[candida]ascalaphidarum_1.fasta/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr25.fasta', <ValueProxy object, typeid 'Value' at 0x7f9f4fe144c0>, <AcquirerProxy object, typeid 'Lock' at 0x7f9f4fe14520>), **{})
error: [Errno 2] No such file or directory: 'Y1_[candida]ascalaphidarum_1.bed/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr28.bed' run(('Y1_[candida]ascalaphidarum_1.fasta/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr28.fasta', <ValueProxy object, typeid 'Value' at 0x7f9f4fe15100>, <AcquirerProxy object, typeid 'Lock' at 0x7f9f4fe15160>), **{})
error: [Errno 2] No such file or directory: 'Y1_[candida]ascalaphidarum_1.bed/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr22.bed' run(('Y1_[candida]ascalaphidarum_1.fasta/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr22.fasta', <ValueProxy object, typeid 'Value' at 0x7f9f4fe0b670>, <AcquirerProxy object, typeid 'Lock' at 0x7f9f4fe0b6d0>), **{})
error: [Errno 2] No such file or directory: 'Y1_[candida]ascalaphidarum_1.bed/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr26.bed' run(('Y1_[candida]ascalaphidarum_1.fasta/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr26.fasta', <ValueProxy object, typeid 'Value' at 0x7f9f4fe142e0>, <AcquirerProxy object, typeid 'Lock' at 0x7f9f4fe14340>), **{})
error: [Errno 2] No such file or directory: 'Y1_[candida]ascalaphidarum_1.bed/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr9.bed' run(('Y1_[candida]ascalaphidarum_1.fasta/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr9.fasta', <ValueProxy object, typeid 'Value' at 0x7f9f4fe0bb20>, <AcquirerProxy object, typeid 'Lock' at 0x7f9f4fe0bb50>), **{})
error: [Errno 2] No such file or directory: 'Y1_[candida]ascalaphidarum_1.bed/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr21.bed' run(('Y1_[candida]ascalaphidarum_1.fasta/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr21.fasta', <ValueProxy object, typeid 'Value' at 0x7f9f4fe0b760>, <AcquirerProxy object, typeid 'Lock' at 0x7f9f4fe0b7c0>), **{})
error: [Errno 2] No such file or directory: 'Y1_[candida]ascalaphidarum_1.bed/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr24.bed' run(('Y1_[candida]ascalaphidarum_1.fasta/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr24.fasta', <ValueProxy object, typeid 'Value' at 0x7f9f4fe143d0>, <AcquirerProxy object, typeid 'Lock' at 0x7f9f4fe14430>), **{})
error: [Errno 2] No such file or directory: 'Y1_[candida]ascalaphidarum_1.bed/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr12.bed' run(('Y1_[candida]ascalaphidarum_1.fasta/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr12.fasta', <ValueProxy object, typeid 'Value' at 0x7f9f4fe0bdf0>, <AcquirerProxy object, typeid 'Lock' at 0x7f9f4fe0be20>), **{})
error: [Errno 2] No such file or directory: 'Y1_[candida]ascalaphidarum_1.bed/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr35.bed' run(('Y1_[candida]ascalaphidarum_1.fasta/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr35.fasta', <ValueProxy object, typeid 'Value' at 0x7f9f4fe0b670>, <AcquirerProxy object, typeid 'Lock' at 0x7f9f4fe0b730>), **{})
error: [Errno 2] No such file or directory: 'Y1_[candida]ascalaphidarum_1.bed/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr33.bed' run(('Y1_[candida]ascalaphidarum_1.fasta/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr33.fasta', <ValueProxy object, typeid 'Value' at 0x7f9f4fe0b580>, <AcquirerProxy object, typeid 'Lock' at 0x7f9f4fe0b5e0>), **{})
error: [Errno 2] No such file or directory: 'Y1_[candida]ascalaphidarum_1.bed/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr36.bed' run(('Y1_[candida]ascalaphidarum_1.fasta/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr36.fasta', <ValueProxy object, typeid 'Value' at 0x7f9f4fe142e0>, <AcquirerProxy object, typeid 'Lock' at 0x7f9f4fe143a0>), **{})
error: [Errno 2] No such file or directory: 'Y1_[candida]ascalaphidarum_1.bed/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr32.bed' run(('Y1_[candida]ascalaphidarum_1.fasta/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr32.fasta', <ValueProxy object, typeid 'Value' at 0x7f9f4fe0bd00>, <AcquirerProxy object, typeid 'Lock' at 0x7f9f4fe0bd60>), **{})
error: [Errno 2] No such file or directory: 'Y1_[candida]ascalaphidarum_1.bed/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr34.bed' run(('Y1_[candida]ascalaphidarum_1.fasta/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr34.fasta', <ValueProxy object, typeid 'Value' at 0x7f9f4fe144c0>, <AcquirerProxy object, typeid 'Lock' at 0x7f9f4fe14580>), **{})
error: [Errno 2] No such file or directory: 'Y1_[candida]ascalaphidarum_1.bed/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr31.bed' run(('Y1_[candida]ascalaphidarum_1.fasta/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr31.fasta', <ValueProxy object, typeid 'Value' at 0x7f9f4fe0bee0>, <AcquirerProxy object, typeid 'Lock' at 0x7f9f4fe0bf40>), **{})
error: [Errno 2] No such file or directory: 'Y1_[candida]ascalaphidarum_1.bed/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr30.bed' run(('Y1_[candida]ascalaphidarum_1.fasta/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr30.fasta', <ValueProxy object, typeid 'Value' at 0x7f9f4fe0b490>, <AcquirerProxy object, typeid 'Lock' at 0x7f9f4fe0b4f0>), **{})
error: [Errno 2] No such file or directory: 'Y1_[candida]ascalaphidarum_1.bed/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr10.bed' run(('Y1_[candida]ascalaphidarum_1.fasta/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr10.fasta', <ValueProxy object, typeid 'Value' at 0x7f9f4fe0bc10>, <AcquirerProxy object, typeid 'Lock' at 0x7f9f4fe0bc40>), **{})
error: [Errno 2] No such file or directory: 'Y1_[candida]ascalaphidarum_1.bed/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr8.bed' run(('Y1_[candida]ascalaphidarum_1.fasta/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr8.fasta', <ValueProxy object, typeid 'Value' at 0x7f9f4fe0ba30>, <AcquirerProxy object, typeid 'Lock' at 0x7f9f4fe0ba60>), **{})
error: [Errno 2] No such file or directory: 'Y1_[candida]ascalaphidarum_1.bed/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr29.bed' run(('Y1_[candida]ascalaphidarum_1.fasta/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr29.fasta', <ValueProxy object, typeid 'Value' at 0x7f9f4fe0bfd0>, <AcquirerProxy object, typeid 'Lock' at 0x7f9f4fe16070>), **{})
error: [Errno 2] No such file or directory: 'Y1_[candida]ascalaphidarum_1.bed/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr7.bed' run(('Y1_[candida]ascalaphidarum_1.fasta/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr7.fasta', <ValueProxy object, typeid 'Value' at 0x7f9f4fe0b940>, <AcquirerProxy object, typeid 'Lock' at 0x7f9f4fe0b970>), **{})
error: [Errno 2] No such file or directory: 'Y1_[candida]ascalaphidarum_1.bed/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr37.bed' run(('Y1_[candida]ascalaphidarum_1.fasta/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr37.fasta', <ValueProxy object, typeid 'Value' at 0x7f9f4fe15100>, <AcquirerProxy object, typeid 'Lock' at 0x7f9f4fe151c0>), **{})
error: [Errno 2] No such file or directory: 'Y1_[candida]ascalaphidarum_1.bed/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr6.bed' run(('Y1_[candida]ascalaphidarum_1.fasta/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr6.fasta', <ValueProxy object, typeid 'Value' at 0x7f9f4fe0b850>, <AcquirerProxy object, typeid 'Lock' at 0x7f9f4fe0b880>), **{})
error: [Errno 2] No such file or directory: 'Y1_[candida]ascalaphidarum_1.bed/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr27.bed' run(('Y1_[candida]ascalaphidarum_1.fasta/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr27.fasta', <ValueProxy object, typeid 'Value' at 0x7f9f4fe141f0>, <AcquirerProxy object, typeid 'Lock' at 0x7f9f4fe14250>), **{})
error: [Errno 2] No such file or directory: 'Y1_[candida]ascalaphidarum_1.bed/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr23.bed' run(('Y1_[candida]ascalaphidarum_1.fasta/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr23.fasta', <ValueProxy object, typeid 'Value' at 0x7f9f4fe145b0>, <AcquirerProxy object, typeid 'Lock' at 0x7f9f4fe14610>), **{})
error: [Errno 2] No such file or directory: 'Y1_[candida]ascalaphidarum_1.bed/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr38.bed' run(('Y1_[candida]ascalaphidarum_1.fasta/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr38.fasta', <ValueProxy object, typeid 'Value' at 0x7f9f4fe0bb20>, <AcquirerProxy object, typeid 'Lock' at 0x7f9f4fe0bb80>), **{})
error: [Errno 2] No such file or directory: 'Y1_[candida]ascalaphidarum_1.bed/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr41.bed' run(('Y1_[candida]ascalaphidarum_1.fasta/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr41.fasta', <ValueProxy object, typeid 'Value' at 0x7f9f4fe0b580>, <AcquirerProxy object, typeid 'Lock' at 0x7f9f4fe0b640>), **{})
error: [Errno 2] No such file or directory: 'Y1_[candida]ascalaphidarum_1.bed/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr39.bed' run(('Y1_[candida]ascalaphidarum_1.fasta/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr39.fasta', <ValueProxy object, typeid 'Value' at 0x7f9f4fe143d0>, <AcquirerProxy object, typeid 'Lock' at 0x7f9f4fe14490>), **{})
error: [Errno 2] No such file or directory: 'Y1_[candida]ascalaphidarum_1.bed/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr40.bed' run(('Y1_[candida]ascalaphidarum_1.fasta/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr40.fasta', <ValueProxy object, typeid 'Value' at 0x7f9f4fe0bdf0>, <AcquirerProxy object, typeid 'Lock' at 0x7f9f4fe0be50>), **{})
error: [Errno 2] No such file or directory: 'Y1_[candida]ascalaphidarum_1.bed/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr42.bed' run(('Y1_[candida]ascalaphidarum_1.fasta/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr42.fasta', <ValueProxy object, typeid 'Value' at 0x7f9f4fe0b670>, <AcquirerProxy object, typeid 'Lock' at 0x7f9f4fe0b6a0>), **{})
error: [Errno 2] No such file or directory: 'Y1_[candida]ascalaphidarum_1.bed/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr43.bed' run(('Y1_[candida]ascalaphidarum_1.fasta/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr43.fasta', <ValueProxy object, typeid 'Value' at 0x7f9f4fe0b760>, <AcquirerProxy object, typeid 'Lock' at 0x7f9f4fe0b820>), **{})
error: [Errno 2] No such file or directory: 'Y1_[candida]ascalaphidarum_1.bed/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr45.bed' run(('Y1_[candida]ascalaphidarum_1.fasta/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr45.fasta', <ValueProxy object, typeid 'Value' at 0x7f9f4fe144c0>, <AcquirerProxy object, typeid 'Lock' at 0x7f9f4fe144f0>), **{})
error: [Errno 2] No such file or directory: 'Y1_[candida]ascalaphidarum_1.bed/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr44.bed' run(('Y1_[candida]ascalaphidarum_1.fasta/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr44.fasta', <ValueProxy object, typeid 'Value' at 0x7f9f4fe142e0>, <AcquirerProxy object, typeid 'Lock' at 0x7f9f4fe14310>), **{})
error: [Errno 2] No such file or directory: 'Y1_[candida]ascalaphidarum_1.bed/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr46.bed' run(('Y1_[candida]ascalaphidarum_1.fasta/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr46.fasta', <ValueProxy object, typeid 'Value' at 0x7f9f4fe0bd00>, <AcquirerProxy object, typeid 'Lock' at 0x7f9f4fe0bdc0>), **{})
error: [Errno 2] No such file or directory: 'Y1_[candida]ascalaphidarum_1.bed/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr47.bed' run(('Y1_[candida]ascalaphidarum_1.fasta/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr47.fasta', <ValueProxy object, typeid 'Value' at 0x7f9f4fe0bee0>, <AcquirerProxy object, typeid 'Lock' at 0x7f9f4fe0bfa0>), **{})
error: [Errno 2] No such file or directory: 'Y1_[candida]ascalaphidarum_1.bed/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr48.bed' run(('Y1_[candida]ascalaphidarum_1.fasta/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr48.fasta', <ValueProxy object, typeid 'Value' at 0x7f9f4fe0bc10>, <AcquirerProxy object, typeid 'Lock' at 0x7f9f4fe0bc70>), **{})
error: [Errno 2] No such file or directory: 'Y1_[candida]ascalaphidarum_1.bed/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr49.bed' run(('Y1_[candida]ascalaphidarum_1.fasta/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr49.fasta', <ValueProxy object, typeid 'Value' at 0x7f9f4fe0b490>, <AcquirerProxy object, typeid 'Lock' at 0x7f9f4fe0b550>), **{})
error: [Errno 2] No such file or directory: 'Y1_[candida]ascalaphidarum_1.bed/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr51.bed' run(('Y1_[candida]ascalaphidarum_1.fasta/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr51.fasta', <ValueProxy object, typeid 'Value' at 0x7f9f4fe0ba30>, <AcquirerProxy object, typeid 'Lock' at 0x7f9f4fe0ba90>), **{})
error: [Errno 2] No such file or directory: 'Y1_[candida]ascalaphidarum_1.bed/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr50.bed' run(('Y1_[candida]ascalaphidarum_1.fasta/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr50.fasta', <ValueProxy object, typeid 'Value' at 0x7f9f4fe0bfd0>, <AcquirerProxy object, typeid 'Lock' at 0x7f9f4fe160d0>), **{})
error: [Errno 2] No such file or directory: 'Y1_[candida]ascalaphidarum_1.bed/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr52.bed' run(('Y1_[candida]ascalaphidarum_1.fasta/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr52.fasta', <ValueProxy object, typeid 'Value' at 0x7f9f4fe0b940>, <AcquirerProxy object, typeid 'Lock' at 0x7f9f4fe0b9a0>), **{})
error: [Errno 2] No such file or directory: 'Y1_[candida]ascalaphidarum_1.bed/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr54.bed' run(('Y1_[candida]ascalaphidarum_1.fasta/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr54.fasta', <ValueProxy object, typeid 'Value' at 0x7f9f4fe0b850>, <AcquirerProxy object, typeid 'Lock' at 0x7f9f4fe0b8b0>), **{})
error: [Errno 2] No such file or directory: 'Y1_[candida]ascalaphidarum_1.bed/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr57.bed' run(('Y1_[candida]ascalaphidarum_1.fasta/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr57.fasta', <ValueProxy object, typeid 'Value' at 0x7f9f4fe0bb20>, <AcquirerProxy object, typeid 'Lock' at 0x7f9f4fe0bbe0>), **{})
error: [Errno 2] No such file or directory: 'Y1_[candida]ascalaphidarum_1.bed/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr55.bed' run(('Y1_[candida]ascalaphidarum_1.fasta/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr55.fasta', <ValueProxy object, typeid 'Value' at 0x7f9f4fe141f0>, <AcquirerProxy object, typeid 'Lock' at 0x7f9f4fe142b0>), **{})
error: [Errno 2] No such file or directory: 'Y1_[candida]ascalaphidarum_1.bed/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr56.bed' run(('Y1_[candida]ascalaphidarum_1.fasta/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr56.fasta', <ValueProxy object, typeid 'Value' at 0x7f9f4fe145b0>, <AcquirerProxy object, typeid 'Lock' at 0x7f9f4fe14670>), **{})
error: [Errno 2] No such file or directory: 'Y1_[candida]ascalaphidarum_1.bed/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr61.bed' run(('Y1_[candida]ascalaphidarum_1.fasta/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr61.fasta', <ValueProxy object, typeid 'Value' at 0x7f9f4fe0b670>, <AcquirerProxy object, typeid 'Lock' at 0x7f9f4fe0b6d0>), **{})
error: [Errno 2] No such file or directory: 'Y1_[candida]ascalaphidarum_1.bed/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr62.bed' run(('Y1_[candida]ascalaphidarum_1.fasta/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr62.fasta', <ValueProxy object, typeid 'Value' at 0x7f9f4fe0b760>, <AcquirerProxy object, typeid 'Lock' at 0x7f9f4fe0b790>), **{})
error: [Errno 2] No such file or directory: 'Y1_[candida]ascalaphidarum_1.bed/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr63.bed' run(('Y1_[candida]ascalaphidarum_1.fasta/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr63.fasta', <ValueProxy object, typeid 'Value' at 0x7f9f4fe142e0>, <AcquirerProxy object, typeid 'Lock' at 0x7f9f4fe14340>), **{})
error: [Errno 2] No such file or directory: 'Y1_[candida]ascalaphidarum_1.bed/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr64.bed' run(('Y1_[candida]ascalaphidarum_1.fasta/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr64.fasta', <ValueProxy object, typeid 'Value' at 0x7f9f4fe144c0>, <AcquirerProxy object, typeid 'Lock' at 0x7f9f4fe14520>), **{})
error: [Errno 2] No such file or directory: 'Y1_[candida]ascalaphidarum_1.bed/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr1.bed' run(('Y1_[candida]ascalaphidarum_1.fasta/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr1.fasta', <ValueProxy object, typeid 'Value' at 0x7f9f4fe0b3a0>, <AcquirerProxy object, typeid 'Lock' at 0x7f9f4fe0b3d0>), **{})
error: [Errno 2] No such file or directory: 'Y1_[candida]ascalaphidarum_1.bed/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr67.bed' run(('Y1_[candida]ascalaphidarum_1.fasta/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr67.fasta', <ValueProxy object, typeid 'Value' at 0x7f9f4fe0bc10>, <AcquirerProxy object, typeid 'Lock' at 0x7f9f4fe0bcd0>), **{})
error: [Errno 2] No such file or directory: 'Y1_[candida]ascalaphidarum_1.bed/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr68.bed' run(('Y1_[candida]ascalaphidarum_1.fasta/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr68.fasta', <ValueProxy object, typeid 'Value' at 0x7f9f4fe0ba30>, <AcquirerProxy object, typeid 'Lock' at 0x7f9f4fe0baf0>), **{})
error: [Errno 2] No such file or directory: 'Y1_[candida]ascalaphidarum_1.bed/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr66.bed' run(('Y1_[candida]ascalaphidarum_1.fasta/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr66.fasta', <ValueProxy object, typeid 'Value' at 0x7f9f4fe0bee0>, <AcquirerProxy object, typeid 'Lock' at 0x7f9f4fe0bf10>), **{})
error: [Errno 2] No such file or directory: 'Y1_[candida]ascalaphidarum_1.bed/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr69.bed' run(('Y1_[candida]ascalaphidarum_1.fasta/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr69.fasta', <ValueProxy object, typeid 'Value' at 0x7f9f4fe0b490>, <AcquirerProxy object, typeid 'Lock' at 0x7f9f4fe0b4c0>), **{})
error: [Errno 2] No such file or directory: 'Y1_[candida]ascalaphidarum_1.bed/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr70.bed' run(('Y1_[candida]ascalaphidarum_1.fasta/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr70.fasta', <ValueProxy object, typeid 'Value' at 0x7f9f4fe0bfd0>, <AcquirerProxy object, typeid 'Lock' at 0x7f9f4fe16040>), **{})
error: [Errno 2] No such file or directory: 'Y1_[candida]ascalaphidarum_1.bed/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr74.bed' run(('Y1_[candida]ascalaphidarum_1.fasta/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr74.fasta', <ValueProxy object, typeid 'Value' at 0x7f9f4fe141f0>, <AcquirerProxy object, typeid 'Lock' at 0x7f9f4fe14220>), **{})
error: [Errno 2] No such file or directory: 'Y1_[candida]ascalaphidarum_1.bed/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr76.bed' run(('Y1_[candida]ascalaphidarum_1.fasta/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr76.fasta', <ValueProxy object, typeid 'Value' at 0x7f9f4fe0b670>, <AcquirerProxy object, typeid 'Lock' at 0x7f9f4fe0b730>), **{})
error: [Errno 2] No such file or directory: 'Y1_[candida]ascalaphidarum_1.bed/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr77.bed' run(('Y1_[candida]ascalaphidarum_1.fasta/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr77.fasta', <ValueProxy object, typeid 'Value' at 0x7f9f4fe0b760>, <AcquirerProxy object, typeid 'Lock' at 0x7f9f4fe0b7c0>), **{})
error: [Errno 2] No such file or directory: 'Y1_[candida]ascalaphidarum_1.bed/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr81.bed' run(('Y1_[candida]ascalaphidarum_1.fasta/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr81.fasta', <ValueProxy object, typeid 'Value' at 0x7f9f4fe0bc10>, <AcquirerProxy object, typeid 'Lock' at 0x7f9f4fe0bc40>), **{})
error: [Errno 2] No such file or directory: 'Y1_[candida]ascalaphidarum_1.bed/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr87.bed' run(('Y1_[candida]ascalaphidarum_1.fasta/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr87.fasta', <ValueProxy object, typeid 'Value' at 0x7f9f4fe0bfd0>, <AcquirerProxy object, typeid 'Lock' at 0x7f9f4fe16070>), **{})
error: [Errno 2] No such file or directory: 'Y1_[candida]ascalaphidarum_1.bed/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr90.bed' run(('Y1_[candida]ascalaphidarum_1.fasta/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr90.fasta', <ValueProxy object, typeid 'Value' at 0x7f9f4fe143d0>, <AcquirerProxy object, typeid 'Lock' at 0x7f9f4fe14430>), **{})
error: [Errno 2] No such file or directory: 'Y1_[candida]ascalaphidarum_1.bed/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr91.bed' run(('Y1_[candida]ascalaphidarum_1.fasta/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr91.fasta', <ValueProxy object, typeid 'Value' at 0x7f9f4fe0b760>, <AcquirerProxy object, typeid 'Lock' at 0x7f9f4fe0b820>), **{})
error: [Errno 2] No such file or directory: 'Y1_[candida]ascalaphidarum_1.bed/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr93.bed' run(('Y1_[candida]ascalaphidarum_1.fasta/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr93.fasta', <ValueProxy object, typeid 'Value' at 0x7f9f4fe0bc10>, <AcquirerProxy object, typeid 'Lock' at 0x7f9f4fe0bc70>), **{})
error: [Errno 2] No such file or directory: 'Y1_[candida]ascalaphidarum_1.bed/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr94.bed' run(('Y1_[candida]ascalaphidarum_1.fasta/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr94.fasta', <ValueProxy object, typeid 'Value' at 0x7f9f4fe0bfd0>, <AcquirerProxy object, typeid 'Lock' at 0x7f9f4fe160d0>), **{})
error: [Errno 2] No such file or directory: 'Y1_[candida]ascalaphidarum_1.bed/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr100.bed' run(('Y1_[candida]ascalaphidarum_1.fasta/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr100.fasta', <ValueProxy object, typeid 'Value' at 0x7f9f4fe0b760>, <AcquirerProxy object, typeid 'Lock' at 0x7f9f4fe0b790>), **{})
error: [Errno 2] No such file or directory: 'Y1_[candida]ascalaphidarum_1.bed/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr101.bed' run(('Y1_[candida]ascalaphidarum_1.fasta/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr101.fasta', <ValueProxy object, typeid 'Value' at 0x7f9f4fe145b0>, <AcquirerProxy object, typeid 'Lock' at 0x7f9f4fe14610>), **{})
error: [Errno 2] No such file or directory: 'Y1_[candida]ascalaphidarum_1.bed/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr105.bed' run(('Y1_[candida]ascalaphidarum_1.fasta/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr105.fasta', <ValueProxy object, typeid 'Value' at 0x7f9f4fe0bfd0>, <AcquirerProxy object, typeid 'Lock' at 0x7f9f4fe16040>), **{})
error: [Errno 2] No such file or directory: 'Y1_[candida]ascalaphidarum_1.bed/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr107.bed' run(('Y1_[candida]ascalaphidarum_1.fasta/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr107.fasta', <ValueProxy object, typeid 'Value' at 0x7f9f4fe0b760>, <AcquirerProxy object, typeid 'Lock' at 0x7f9f4fe0b7c0>), **{})
error: [Errno 2] No such file or directory: 'Y1_[candida]ascalaphidarum_1.bed/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr109.bed' run(('Y1_[candida]ascalaphidarum_1.fasta/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr109.fasta', <ValueProxy object, typeid 'Value' at 0x7f9f4fe0b3a0>, <AcquirerProxy object, typeid 'Lock' at 0x7f9f4fe0b460>), **{})
error: [Errno 2] No such file or directory: 'Y1_[candida]ascalaphidarum_1.bed/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr111.bed' run(('Y1_[candida]ascalaphidarum_1.fasta/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr111.fasta', <ValueProxy object, typeid 'Value' at 0x7f9f4fe0b490>, <AcquirerProxy object, typeid 'Lock' at 0x7f9f4fe0b550>), **{})
error: [Errno 2] No such file or directory: 'Y1_[candida]ascalaphidarum_1.bed/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr112.bed' run(('Y1_[candida]ascalaphidarum_1.fasta/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr112.fasta', <ValueProxy object, typeid 'Value' at 0x7f9f4fe0bfd0>, <AcquirerProxy object, typeid 'Lock' at 0x7f9f4fe16070>), **{})
error: [Errno 2] No such file or directory: 'Y1_[candida]ascalaphidarum_1.bed/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr113.bed' run(('Y1_[candida]ascalaphidarum_1.fasta/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr113.fasta', <ValueProxy object, typeid 'Value' at 0x7f9f4fe0b580>, <AcquirerProxy object, typeid 'Lock' at 0x7f9f4fe0b640>), **{})
error: [Errno 2] No such file or directory: 'Y1_[candida]ascalaphidarum_1.bed/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr117.bed' run(('Y1_[candida]ascalaphidarum_1.fasta/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr117.fasta', <ValueProxy object, typeid 'Value' at 0x7f9f4fe0b3a0>, <AcquirerProxy object, typeid 'Lock' at 0x7f9f4fe0b3d0>), **{})
error: [Errno 2] No such file or directory: 'Y1_[candida]ascalaphidarum_1.bed/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr118.bed' run(('Y1_[candida]ascalaphidarum_1.fasta/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr118.fasta', <ValueProxy object, typeid 'Value' at 0x7f9f4fe0b670>, <AcquirerProxy object, typeid 'Lock' at 0x7f9f4fe0b6d0>), **{})
error: [Errno 2] No such file or directory: 'Y1_[candida]ascalaphidarum_1.bed/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr119.bed' run(('Y1_[candida]ascalaphidarum_1.fasta/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr119.fasta', <ValueProxy object, typeid 'Value' at 0x7f9f4fe0b490>, <AcquirerProxy object, typeid 'Lock' at 0x7f9f4fe0b4c0>), **{})
error: [Errno 2] No such file or directory: 'Y1_[candida]ascalaphidarum_1.bed/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr120.bed' run(('Y1_[candida]ascalaphidarum_1.fasta/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr120.fasta', <ValueProxy object, typeid 'Value' at 0x7f9f4fe0bdf0>, <AcquirerProxy object, typeid 'Lock' at 0x7f9f4fe0be50>), **{})
error: [Errno 2] No such file or directory: 'Y1_[candida]ascalaphidarum_1.bed/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr121.bed' run(('Y1_[candida]ascalaphidarum_1.fasta/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr121.fasta', <ValueProxy object, typeid 'Value' at 0x7f9f4fe0bfd0>, <AcquirerProxy object, typeid 'Lock' at 0x7f9f4fe160d0>), **{})
error: [Errno 2] No such file or directory: 'Y1_[candida]ascalaphidarum_1.bed/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr123.bed' run(('Y1_[candida]ascalaphidarum_1.fasta/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr123.fasta', <ValueProxy object, typeid 'Value' at 0x7f9f4fe0bd00>, <AcquirerProxy object, typeid 'Lock' at 0x7f9f4fe0bdc0>), **{})
error: [Errno 2] No such file or directory: 'Y1_[candida]ascalaphidarum_1.bed/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr127.bed' run(('Y1_[candida]ascalaphidarum_1.fasta/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr127.fasta', <ValueProxy object, typeid 'Value' at 0x7f9f4fe0b490>, <AcquirerProxy object, typeid 'Lock' at 0x7f9f4fe0b4f0>), **{})
error: [Errno 2] No such file or directory: 'Y1_[candida]ascalaphidarum_1.bed/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr128.bed' run(('Y1_[candida]ascalaphidarum_1.fasta/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr128.fasta', <ValueProxy object, typeid 'Value' at 0x7f9f4fe0bdf0>, <AcquirerProxy object, typeid 'Lock' at 0x7f9f4fe0beb0>), **{})
error: [Errno 2] No such file or directory: 'Y1_[candida]ascalaphidarum_1.bed/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr130.bed' run(('Y1_[candida]ascalaphidarum_1.fasta/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr130.fasta', <ValueProxy object, typeid 'Value' at 0x7f9f4fe0b940>, <AcquirerProxy object, typeid 'Lock' at 0x7f9f4fe0b9a0>), **{})
error: [Errno 2] No such file or directory: 'Y1_[candida]ascalaphidarum_1.bed/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr131.bed' run(('Y1_[candida]ascalaphidarum_1.fasta/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr131.fasta', <ValueProxy object, typeid 'Value' at 0x7f9f4fe0bd00>, <AcquirerProxy object, typeid 'Lock' at 0x7f9f4fe0bd30>), **{})
error: [Errno 2] No such file or directory: 'Y1_[candida]ascalaphidarum_1.bed/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr133.bed' run(('Y1_[candida]ascalaphidarum_1.fasta/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr133.fasta', <ValueProxy object, typeid 'Value' at 0x7f9f4fe0b490>, <AcquirerProxy object, typeid 'Lock' at 0x7f9f4fe0b550>), **{})
error: [Errno 2] No such file or directory: 'Y1_[candida]ascalaphidarum_1.bed/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr134.bed' run(('Y1_[candida]ascalaphidarum_1.fasta/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr134.fasta', <ValueProxy object, typeid 'Value' at 0x7f9f4fe0bdf0>, <AcquirerProxy object, typeid 'Lock' at 0x7f9f4fe0be20>), **{})
error: [Errno 2] No such file or directory: 'Y1_[candida]ascalaphidarum_1.bed/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr135.bed' run(('Y1_[candida]ascalaphidarum_1.fasta/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr135.fasta', <ValueProxy object, typeid 'Value' at 0x7f9f4fe0bb20>, <AcquirerProxy object, typeid 'Lock' at 0x7f9f4fe0bbe0>), **{})
error: [Errno 2] No such file or directory: 'Y1_[candida]ascalaphidarum_1.bed/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr137.bed' run(('Y1_[candida]ascalaphidarum_1.fasta/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr137.fasta', <ValueProxy object, typeid 'Value' at 0x7f9f4fe0b940>, <AcquirerProxy object, typeid 'Lock' at 0x7f9f4fe0ba00>), **{})
error: [Errno 2] No such file or directory: 'Y1_[candida]ascalaphidarum_1.bed/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr138.bed' run(('Y1_[candida]ascalaphidarum_1.fasta/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr138.fasta', <ValueProxy object, typeid 'Value' at 0x7f9f4fe0bd00>, <AcquirerProxy object, typeid 'Lock' at 0x7f9f4fe0bd60>), **{})
error: [Errno 2] No such file or directory: 'Y1_[candida]ascalaphidarum_1.bed/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr140.bed' run(('Y1_[candida]ascalaphidarum_1.fasta/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr140.fasta', <ValueProxy object, typeid 'Value' at 0x7f9f4fe142e0>, <AcquirerProxy object, typeid 'Lock' at 0x7f9f4fe14340>), **{})
error: [Errno 2] No such file or directory: 'Y1_[candida]ascalaphidarum_1.bed/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr141.bed' run(('Y1_[candida]ascalaphidarum_1.fasta/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr141.fasta', <ValueProxy object, typeid 'Value' at 0x7f9f4fe0bb20>, <AcquirerProxy object, typeid 'Lock' at 0x7f9f4fe0bb50>), **{})
error: [Errno 2] No such file or directory: 'Y1_[candida]ascalaphidarum_1.bed/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr143.bed' run(('Y1_[candida]ascalaphidarum_1.fasta/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr143.fasta', <ValueProxy object, typeid 'Value' at 0x7f9f4fe15100>, <AcquirerProxy object, typeid 'Lock' at 0x7f9f4fe15130>), **{})
error: [Errno 2] No such file or directory: 'Y1_[candida]ascalaphidarum_1.bed/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr144.bed' run(('Y1_[candida]ascalaphidarum_1.fasta/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr144.fasta', <ValueProxy object, typeid 'Value' at 0x7f9f4fe0bdf0>, <AcquirerProxy object, typeid 'Lock' at 0x7f9f4fe0be50>), **{})
error: [Errno 2] No such file or directory: 'Y1_[candida]ascalaphidarum_1.bed/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr146.bed' run(('Y1_[candida]ascalaphidarum_1.fasta/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr146.fasta', <ValueProxy object, typeid 'Value' at 0x7f9f4fe0bd00>, <AcquirerProxy object, typeid 'Lock' at 0x7f9f4fe0bdc0>), **{})
error: [Errno 2] No such file or directory: 'Y1_[candida]ascalaphidarum_1.bed/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr152.bed' run(('Y1_[candida]ascalaphidarum_1.fasta/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr152.fasta', <ValueProxy object, typeid 'Value' at 0x7f9f4fe0bee0>, <AcquirerProxy object, typeid 'Lock' at 0x7f9f4fe0bf10>), **{})
error: [Errno 2] No such file or directory: 'Y1_[candida]ascalaphidarum_1.bed/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr154.bed' run(('Y1_[candida]ascalaphidarum_1.fasta/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr154.fasta', <ValueProxy object, typeid 'Value' at 0x7f9f4fe0bd00>, <AcquirerProxy object, typeid 'Lock' at 0x7f9f4fe0bd30>), **{})
error: [Errno 2] No such file or directory: 'Y1_[candida]ascalaphidarum_1.bed/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr156.bed' run(('Y1_[candida]ascalaphidarum_1.fasta/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr156.fasta', <ValueProxy object, typeid 'Value' at 0x7f9f4fe141f0>, <AcquirerProxy object, typeid 'Lock' at 0x7f9f4fe14220>), **{})
error: [Errno 2] No such file or directory: 'Y1_[candida]ascalaphidarum_1.bed/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr158.bed' run(('Y1_[candida]ascalaphidarum_1.fasta/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr158.fasta', <ValueProxy object, typeid 'Value' at 0x7f9f4fe0bee0>, <AcquirerProxy object, typeid 'Lock' at 0x7f9f4fe0bf40>), **{})
error: [Errno 2] No such file or directory: 'Y1_[candida]ascalaphidarum_1.bed/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr160.bed' run(('Y1_[candida]ascalaphidarum_1.fasta/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr160.fasta', <ValueProxy object, typeid 'Value' at 0x7f9f4fe0b3a0>, <AcquirerProxy object, typeid 'Lock' at 0x7f9f4fe0b460>), **{})
error: [Errno 2] No such file or directory: 'Y1_[candida]ascalaphidarum_1.bed/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr166.bed' run(('Y1_[candida]ascalaphidarum_1.fasta/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr166.fasta', <ValueProxy object, typeid 'Value' at 0x7f9f4fe0b3a0>, <AcquirerProxy object, typeid 'Lock' at 0x7f9f4fe0b3d0>), **{})
error: [Errno 2] No such file or directory: 'Y1_[candida]ascalaphidarum_1.bed/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr169.bed' run(('Y1_[candida]ascalaphidarum_1.fasta/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr169.fasta', <ValueProxy object, typeid 'Value' at 0x7f9f4fe0b490>, <AcquirerProxy object, typeid 'Lock' at 0x7f9f4fe0b4f0>), **{})
error: [Errno 2] No such file or directory: 'Y1_[candida]ascalaphidarum_1.bed/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr173.bed' run(('Y1_[candida]ascalaphidarum_1.fasta/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr173.fasta', <ValueProxy object, typeid 'Value' at 0x7f9f4fe0ba30>, <AcquirerProxy object, typeid 'Lock' at 0x7f9f4fe0ba60>), **{})
error: [Errno 2] No such file or directory: 'Y1_[candida]ascalaphidarum_1.bed/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr175.bed' run(('Y1_[candida]ascalaphidarum_1.fasta/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr175.fasta', <ValueProxy object, typeid 'Value' at 0x7f9f4fe142e0>, <AcquirerProxy object, typeid 'Lock' at 0x7f9f4fe14310>), **{})
error: [Errno 2] No such file or directory: 'Y1_[candida]ascalaphidarum_1.bed/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr174.bed' run(('Y1_[candida]ascalaphidarum_1.fasta/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr174.fasta', <ValueProxy object, typeid 'Value' at 0x7f9f4fe145b0>, <AcquirerProxy object, typeid 'Lock' at 0x7f9f4fe14610>), **{})
error: [Errno 2] No such file or directory: 'Y1_[candida]ascalaphidarum_1.bed/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr178.bed' run(('Y1_[candida]ascalaphidarum_1.fasta/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr178.fasta', <ValueProxy object, typeid 'Value' at 0x7f9f4fe15100>, <AcquirerProxy object, typeid 'Lock' at 0x7f9f4fe151c0>), **{})
error: [Errno 2] No such file or directory: 'Y1_[candida]ascalaphidarum_1.bed/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr181.bed' run(('Y1_[candida]ascalaphidarum_1.fasta/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr181.fasta', <ValueProxy object, typeid 'Value' at 0x7f9f4fe0bdf0>, <AcquirerProxy object, typeid 'Lock' at 0x7f9f4fe0be20>), **{})
error: [Errno 2] No such file or directory: 'Y1_[candida]ascalaphidarum_1.bed/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr182.bed' run(('Y1_[candida]ascalaphidarum_1.fasta/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr182.fasta', <ValueProxy object, typeid 'Value' at 0x7f9f4fe145b0>, <AcquirerProxy object, typeid 'Lock' at 0x7f9f4fe14670>), **{})
error: [Errno 2] No such file or directory: 'Y1_[candida]ascalaphidarum_1.bed/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr183.bed' run(('Y1_[candida]ascalaphidarum_1.fasta/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr183.fasta', <ValueProxy object, typeid 'Value' at 0x7f9f4fe0b760>, <AcquirerProxy object, typeid 'Lock' at 0x7f9f4fe0b7c0>), **{})
error: [Errno 2] No such file or directory: 'Y1_[candida]ascalaphidarum_1.bed/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr185.bed' run(('Y1_[candida]ascalaphidarum_1.fasta/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr185.fasta', <ValueProxy object, typeid 'Value' at 0x7f9f4fe0b580>, <AcquirerProxy object, typeid 'Lock' at 0x7f9f4fe0b640>), **{})
error: [Errno 2] No such file or directory: 'Y1_[candida]ascalaphidarum_1.bed/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr188.bed' run(('Y1_[candida]ascalaphidarum_1.fasta/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr188.fasta', <ValueProxy object, typeid 'Value' at 0x7f9f4fe0b670>, <AcquirerProxy object, typeid 'Lock' at 0x7f9f4fe0b6d0>), **{})
error: [Errno 2] No such file or directory: 'Y1_[candida]ascalaphidarum_1.bed/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr189.bed' run(('Y1_[candida]ascalaphidarum_1.fasta/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr189.fasta', <ValueProxy object, typeid 'Value' at 0x7f9f4fe0b760>, <AcquirerProxy object, typeid 'Lock' at 0x7f9f4fe0b820>), **{})
error: [Errno 2] No such file or directory: 'Y1_[candida]ascalaphidarum_1.bed/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr191.bed' run(('Y1_[candida]ascalaphidarum_1.fasta/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr191.fasta', <ValueProxy object, typeid 'Value' at 0x7f9f4fe0bd00>, <AcquirerProxy object, typeid 'Lock' at 0x7f9f4fe0bdc0>), **{})
error: [Errno 2] No such file or directory: 'Y1_[candida]ascalaphidarum_1.bed/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr194.bed' run(('Y1_[candida]ascalaphidarum_1.fasta/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr194.fasta', <ValueProxy object, typeid 'Value' at 0x7f9f4fe0b670>, <AcquirerProxy object, typeid 'Lock' at 0x7f9f4fe0b730>), **{})
error: [Errno 2] No such file or directory: 'Y1_[candida]ascalaphidarum_1.bed/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr196.bed' run(('Y1_[candida]ascalaphidarum_1.fasta/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr196.fasta', <ValueProxy object, typeid 'Value' at 0x7f9f4fe0b850>, <AcquirerProxy object, typeid 'Lock' at 0x7f9f4fe0b880>), **{})
error: [Errno 2] No such file or directory: 'Y1_[candida]ascalaphidarum_1.bed/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr195.bed' run(('Y1_[candida]ascalaphidarum_1.fasta/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr195.fasta', <ValueProxy object, typeid 'Value' at 0x7f9f4fe0b760>, <AcquirerProxy object, typeid 'Lock' at 0x7f9f4fe0b790>), **{})
error: [Errno 2] No such file or directory: 'Y1_[candida]ascalaphidarum_1.bed/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr197.bed' run(('Y1_[candida]ascalaphidarum_1.fasta/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr197.fasta', <ValueProxy object, typeid 'Value' at 0x7f9f4fe0bd00>, <AcquirerProxy object, typeid 'Lock' at 0x7f9f4fe0bd30>), **{})
error: [Errno 2] No such file or directory: 'Y1_[candida]ascalaphidarum_1.bed/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr198.bed' run(('Y1_[candida]ascalaphidarum_1.fasta/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr198.fasta', <ValueProxy object, typeid 'Value' at 0x7f9f4fe0bfd0>, <AcquirerProxy object, typeid 'Lock' at 0x7f9f4fe160d0>), **{})
error: [Errno 2] No such file or directory: 'Y1_[candida]ascalaphidarum_1.bed/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr199.bed' run(('Y1_[candida]ascalaphidarum_1.fasta/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr199.fasta', <ValueProxy object, typeid 'Value' at 0x7f9f4fe0b670>, <AcquirerProxy object, typeid 'Lock' at 0x7f9f4fe0b6a0>), **{})
error: [Errno 2] No such file or directory: 'Y1_[candida]ascalaphidarum_1.bed/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr202.bed' run(('Y1_[candida]ascalaphidarum_1.fasta/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr202.fasta', <ValueProxy object, typeid 'Value' at 0x7f9f4fe144c0>, <AcquirerProxy object, typeid 'Lock' at 0x7f9f4fe144f0>), **{})
error: [Errno 2] No such file or directory: 'Y1_[candida]ascalaphidarum_1.bed/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr203.bed' run(('Y1_[candida]ascalaphidarum_1.fasta/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr203.fasta', <ValueProxy object, typeid 'Value' at 0x7f9f4fe0b760>, <AcquirerProxy object, typeid 'Lock' at 0x7f9f4fe0b7c0>), **{})
error: [Errno 2] No such file or directory: 'Y1_[candida]ascalaphidarum_1.bed/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr205.bed' run(('Y1_[candida]ascalaphidarum_1.fasta/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr205.fasta', <ValueProxy object, typeid 'Value' at 0x7f9f4fe0bc10>, <AcquirerProxy object, typeid 'Lock' at 0x7f9f4fe0bcd0>), **{})
error: [Errno 2] No such file or directory: 'Y1_[candida]ascalaphidarum_1.bed/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr204.bed' run(('Y1_[candida]ascalaphidarum_1.fasta/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr204.fasta', <ValueProxy object, typeid 'Value' at 0x7f9f4fe0bd00>, <AcquirerProxy object, typeid 'Lock' at 0x7f9f4fe0bd60>), **{})
error: [Errno 2] No such file or directory: 'Y1_[candida]ascalaphidarum_1.bed/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr206.bed' run(('Y1_[candida]ascalaphidarum_1.fasta/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr206.fasta', <ValueProxy object, typeid 'Value' at 0x7f9f4fe0bfd0>, <AcquirerProxy object, typeid 'Lock' at 0x7f9f4fe16040>), **{})
error: [Errno 2] No such file or directory: 'Y1_[candida]ascalaphidarum_1.bed/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr209.bed' run(('Y1_[candida]ascalaphidarum_1.fasta/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr209.fasta', <ValueProxy object, typeid 'Value' at 0x7f9f4fe0b940>, <AcquirerProxy object, typeid 'Lock' at 0x7f9f4fe0ba00>), **{})
error: [Errno 2] No such file or directory: 'Y1_[candida]ascalaphidarum_1.bed/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr210.bed' run(('Y1_[candida]ascalaphidarum_1.fasta/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr210.fasta', <ValueProxy object, typeid 'Value' at 0x7f9f4fe144c0>, <AcquirerProxy object, typeid 'Lock' at 0x7f9f4fe14520>), **{})
error: [Errno 2] No such file or directory: 'Y1_[candida]ascalaphidarum_1.bed/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr211.bed' run(('Y1_[candida]ascalaphidarum_1.fasta/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr211.fasta', <ValueProxy object, typeid 'Value' at 0x7f9f4fe0bb20>, <AcquirerProxy object, typeid 'Lock' at 0x7f9f4fe0bb50>), **{})
error: [Errno 2] No such file or directory: 'Y1_[candida]ascalaphidarum_1.bed/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr213.bed' run(('Y1_[candida]ascalaphidarum_1.fasta/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr213.fasta', <ValueProxy object, typeid 'Value' at 0x7f9f4fe0bc10>, <AcquirerProxy object, typeid 'Lock' at 0x7f9f4fe0bc40>), **{})
error: [Errno 2] No such file or directory: 'Y1_[candida]ascalaphidarum_1.bed/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr214.bed' run(('Y1_[candida]ascalaphidarum_1.fasta/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr214.fasta', <ValueProxy object, typeid 'Value' at 0x7f9f4fe0bd00>, <AcquirerProxy object, typeid 'Lock' at 0x7f9f4fe0bdc0>), **{})
error: [Errno 2] No such file or directory: 'Y1_[candida]ascalaphidarum_1.bed/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr215.bed' run(('Y1_[candida]ascalaphidarum_1.fasta/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr215.fasta', <ValueProxy object, typeid 'Value' at 0x7f9f4fe0bfd0>, <AcquirerProxy object, typeid 'Lock' at 0x7f9f4fe16070>), **{})
error: [Errno 2] No such file or directory: 'Y1_[candida]ascalaphidarum_1.bed/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr216.bed' run(('Y1_[candida]ascalaphidarum_1.fasta/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr216.fasta', <ValueProxy object, typeid 'Value' at 0x7f9f4fe0b490>, <AcquirerProxy object, typeid 'Lock' at 0x7f9f4fe0b4c0>), **{})
error: [Errno 2] No such file or directory: 'Y1_[candida]ascalaphidarum_1.bed/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr217.bed' run(('Y1_[candida]ascalaphidarum_1.fasta/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr217.fasta', <ValueProxy object, typeid 'Value' at 0x7f9f4fe0b940>, <AcquirerProxy object, typeid 'Lock' at 0x7f9f4fe0b970>), **{})
error: [Errno 2] No such file or directory: 'Y1_[candida]ascalaphidarum_1.bed/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr218.bed' run(('Y1_[candida]ascalaphidarum_1.fasta/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr218.fasta', <ValueProxy object, typeid 'Value' at 0x7f9f4fe144c0>, <AcquirerProxy object, typeid 'Lock' at 0x7f9f4fe14580>), **{})
error: [Errno 2] No such file or directory: 'Y1_[candida]ascalaphidarum_1.bed/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr219.bed' run(('Y1_[candida]ascalaphidarum_1.fasta/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr219.fasta', <ValueProxy object, typeid 'Value' at 0x7f9f4fe0ba30>, <AcquirerProxy object, typeid 'Lock' at 0x7f9f4fe0baf0>), **{})
error: [Errno 2] No such file or directory: 'Y1_[candida]ascalaphidarum_1.bed/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr220.bed' run(('Y1_[candida]ascalaphidarum_1.fasta/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr220.fasta', <ValueProxy object, typeid 'Value' at 0x7f9f4fe0bb20>, <AcquirerProxy object, typeid 'Lock' at 0x7f9f4fe0bb80>), **{})
error: [Errno 2] No such file or directory: 'Y1_[candida]ascalaphidarum_1.bed/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr221.bed' run(('Y1_[candida]ascalaphidarum_1.fasta/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr221.fasta', <ValueProxy object, typeid 'Value' at 0x7f9f4fe0bc10>, <AcquirerProxy object, typeid 'Lock' at 0x7f9f4fe0bc70>), **{})
error: [Errno 2] No such file or directory: 'Y1_[candida]ascalaphidarum_1.bed/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr222.bed' run(('Y1_[candida]ascalaphidarum_1.fasta/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr222.fasta', <ValueProxy object, typeid 'Value' at 0x7f9f4fe142e0>, <AcquirerProxy object, typeid 'Lock' at 0x7f9f4fe143a0>), **{})
error: [Errno 2] No such file or directory: 'Y1_[candida]ascalaphidarum_1.bed/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr227.bed' run(('Y1_[candida]ascalaphidarum_1.fasta/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr227.fasta', <ValueProxy object, typeid 'Value' at 0x7f9f4fe0b490>, <AcquirerProxy object, typeid 'Lock' at 0x7f9f4fe0b4f0>), **{})
error: [Errno 2] No such file or directory: 'Y1_[candida]ascalaphidarum_1.bed/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr228.bed' run(('Y1_[candida]ascalaphidarum_1.fasta/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr228.fasta', <ValueProxy object, typeid 'Value' at 0x7f9f4fe144c0>, <AcquirerProxy object, typeid 'Lock' at 0x7f9f4fe144f0>), **{})
error: [Errno 2] No such file or directory: 'Y1_[candida]ascalaphidarum_1.bed/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr229.bed' run(*('Y1_[candida]ascalaphidarum_1.fasta/mask_5fbcf89c-496f-479d-8e38-8a12ac177e61/Chr229.fasta', <ValueProxy object, typeid 'Value' at 0x7f9f4fe0bdf0>, <AcquirerProxy object, typeid 'Lock' at 0x7f9f4fe0beb0>), **{})
[Sep 28 02:53 PM]: Genome loaded: 229 scaffolds;
10,463,717 bp;
0.00% repeats masked
[Sep 28 02:53 PM]: Mapping 667,688 proteins to genome using diamond and exonerate
[Sep 28 02:55 PM]: Found 394,199 preliminary alignments with diamond in 0:01:26 --> generated FASTA files for exonerate in 0:00:41
Traceback (most recent call last):
File "/home/workstation/miniconda3/envs/funannotate/lib/python3.8/site-packages/funannotate/aux_scripts/funannotate-p2g.py", line 358, in
funannotate-predict.log
[09/28/22 14:53:33]: /home/workstation/miniconda3/envs/funannotate/bin/funannotate predict -i ../0_B_Repeatmask_sequences/genome_3.fasta -s Y1_[candida]ascalaphidarum_1 -o Y1_[candida]ascalaphidarum_1.fasta --protein_evidence /home/workstation/Genomas_Pipeline_Annotation_FROM_INITIAL_GENOME_ANNOTATION_PIPELINE_SACCHAROMYCOTINA/0_0_DB_FINAL/reference_proteins.fasta /home/workstation/funannotate_db/uniprot_sprot.fasta --force --cpus 20
[09/28/22 14:53:33]: OS: Ubuntu 22.04, 20 cores, ~ 66 GB RAM. Python: 3.8.13 [09/28/22 14:53:33]: Running funannotate v1.8.13 [09/28/22 14:53:34]: GeneMark path: /home/workstation/Programs_software/gmes_linux_64 [09/28/22 14:53:34]: Full path to gmes_petap.pl: /home/workstation/Programs_software/gmes_linux_64/gmes_petap.pl [09/28/22 14:53:34]: GeneMark appears to be functional? False [09/28/22 14:53:34]: exonerate version=exonerate 2.4.0 path=/home/workstation/miniconda3/envs/funannotate/bin/exonerate [09/28/22 14:53:34]: diamond version=2.0.15 path=/home/workstation/miniconda3/envs/funannotate/bin/diamond [09/28/22 14:53:34]: tbl2asn version=no way to determine, likely 25.X path=/home/workstation/miniconda3/envs/funannotate/bin/tbl2asn [09/28/22 14:53:34]: bedtools version=bedtools v2.30.0 path=/home/workstation/miniconda3/envs/funannotate/bin/bedtools [09/28/22 14:53:34]: augustus version=3.4.0 path=/home/workstation/miniconda3/envs/funannotate/bin/augustus [09/28/22 14:53:34]: etraining version=NA path=/home/workstation/miniconda3/envs/funannotate/bin/etraining [09/28/22 14:53:34]: tRNAscan-SE version=2.0.9 (July 2021) path=/home/workstation/miniconda3/envs/funannotate/bin/tRNAscan-SE [09/28/22 14:53:34]: bam2hints version=NA path=/home/workstation/miniconda3/envs/funannotate/bin/bam2hints [09/28/22 14:53:34]: minimap2 version=2.24-r1122 path=/home/workstation/miniconda3/envs/funannotate/bin/minimap2 [09/28/22 14:53:34]: $AUGUSTUS_CONFIG_PATH=/home/workstation/miniconda3/envs/funannotate/config/ [09/28/22 14:53:34]: {'augustus': 1, 'hiq': 2, 'genemark': 0, 'pasa': 6, 'codingquarry': 0, 'snap': 1, 'glimmerhmm': 1, 'proteins': 1, 'transcripts': 1} [09/28/22 14:53:34]: Skipping CodingQuarry as no --rna_bam passed [09/28/22 14:53:34]: {'augustus': 'busco', 'snap': 'busco', 'glimmerhmm': 'busco'} [09/28/22 14:53:34]: Parsed training data, run ab-initio gene predictors as follows: [09/28/22 14:53:35]: {'augustus': 1, 'hiq': 2, 'genemark': 0, 'pasa': 6, 'codingquarry': 0, 'snap': 1, 'glimmerhmm': 1, 'proteins': 1, 'transcripts': 1} [09/28/22 14:53:38]: Loading genome assembly and parsing soft-masked repetitive sequences [09/28/22 14:53:38]: Genome loaded: 229 scaffolds; 10,463,717 bp; 0.00% repeats masked
funannotate-p2g.log
[09/28/22 14:53:50]: /home/workstation/miniconda3/envs/funannotate/lib/python3.8/site-packages/funannotate/aux_scripts/funannotate-p2g.py -p Y1_[candida]ascalaphidarum_1.fasta/predict_misc/proteins.combined.fa -g /home/workstation/Genomas_Pipeline_Annotation_FROM_INITIAL_GENOME_ANNOTATION_PIPELINE_SACCHAROMYCOTINA/0_0_DB_FINAL/Y1_[candida]ascalaphidarum_1/0_C_Predict_annotation_based_on_protein_reference/Y1_[candida]ascalaphidarum_1.fasta/predict_misc/genome.softmasked.fa -o Y1_[candida]ascalaphidarum_1.fasta/predict_misc/protein_alignments.gff3 --maxintron 3000 --cpus 20 --exonerate_pident 80 --ploidy 1 -f diamond --tmpdir /tmp --tblastn_out Y1_[candida]ascalaphidarum_1.fasta/predict_misc/p2g.diamond.out --logfile Y1_[candida]ascalaphidarum_1.fasta/logfiles/funannotate-p2g.log
[09/28/22 14:53:50]: Mapping 667,688 proteins to genome using diamond and exonerate [09/28/22 14:53:50]: Diamond v2.0.15; Exonerate v2.4.0 [09/28/22 14:53:50]: diamond makedb --threads 20 --in /home/workstation/Genomas_Pipeline_Annotation_FROM_INITIAL_GENOME_ANNOTATION_PIPELINE_SACCHAROMYCOTINA/0_0_DB_FINAL/Y1_[candida]ascalaphidarum_1/0_C_Predict_annotation_based_on_protein_reference/Y1_[candida]ascalaphidarum_1.fasta/predict_misc/proteins.combined.fa --db diamond [09/28/22 14:53:53]: diamond blastx --threads 20 -q /home/workstation/Genomas_Pipeline_Annotation_FROM_INITIAL_GENOME_ANNOTATION_PIPELINE_SACCHAROMYCOTINA/0_0_DB_FINAL/Y1_[candida]ascalaphidarum_1/0_C_Predict_annotation_based_on_protein_reference/Y1_[candida]ascalaphidarum_1.fasta/predict_misc/genome.softmasked.fa --db diamond -o diamond.matches.tab -e 1e-10 -k 0 --more-sensitive --unal 0 -c 1 -F 15 -f 6 sseqid slen sstart send qseqid qlen qstart qend pident length evalue score qcovhsp qframe [09/28/22 14:55:53]: Writing 394199 contig splits for exonerate [09/28/22 14:55:59]: Found 394,199 preliminary alignments with diamond in 0:01:26 --> generated FASTA files for exonerate in 0:00:41
In future please try to truncate the log you provide to the necessary info or attach it as a file, this makes for a long scroll...
I would recommend not including the [] in the file names, this might confuse some UNIX programs - not sure every filename is escaped when being sent to the external programs by python.
I'm not sure this is exactly the problem but can you try to run a test run where you don't use '[]' in the species names or the output folders first and see if that allows the masking or masking summary to succeed? Maybe we can protect that code better for having wildcards in the input / output names too but I want to know if this is the cause first before digging into other code.
Hi Jason,
You are right, the latest funannotate version, 1.8.13, have a problem with the “[“ and “]” characters.
In fact, I have given a thought that there could be an incompatibility of “[“ and “]” characters with funannotate.
But I have dismissed that possibility since I ran the same script in another workstation where I have funannotate installed and there was no problem.
However, the funannotate version installed in this other workstation was version 1.8.9.
So, it seems that funannotate version 1.8.9 can parse well the “[“ and “]” characters and pass it to the downstream software but fails to do so when version 1.8.13 is used instead.
Thank you,
Best regards,
Paulo Dias
Hi Funannotate team,
I have spoke too early, although I have removed the "[" and "]" characters, the same error occurred:
[Oct 03 03:15 PM]: Found 394,199 preliminary alignments with diamond in 0:01:24 --> generated FASTA files for exonerate in 0:00:41
Traceback (most recent call last):
File "/home/workstation/miniconda3/envs/funannotate/lib/python3.8/site-packages/funannotate/aux_scripts/funannotate-p2g.py", line 358, in
I encountered the same problem and it was because there was a .fa in my output name. You can see it's being replaced by .bed in your logs, e.g. 'Y1_[candida]ascalaphidarum_1.bed/
