NOVOPlasty icon indicating copy to clipboard operation
NOVOPlasty copied to clipboard

Input data metrics explanation, % of mtDNA reads of the total sequence reads that mapped to the whole mtDNA_follow up

Open AliBasuony2022 opened this issue 5 months ago • 8 comments

Dear Nicolas,

Just a follow up question for the issue #216

How do I know the right % of mtDNA reads of the total sequence reads that mapped to the whole mtDNA? I'm doing a comparison between the performance of NOVOPlasty and other de novo assemblies and this information is so important.

When I used adifferent memmory settings (all other settings are fixed), I have got the same lenght of the largest contig, but with differnt number for assembled reads, aligned and total reads.

Does the subsampled fraction: 99.99 % when setting the Max memory= Null is right? if so, the number of total reads is over the number of reads in the raw data. I'm still confused, sorry.

max memory Null log_mito_1_375_12_6_max memory Null.txt

max memory 100 log_mito_1_375_12_3_max memory 100.txt

memory 64 log_mito_1_375_12_max memory 64.txt

Kind regards, Ali

AliBasuony2022 avatar Feb 06 '24 18:02 AliBasuony2022