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only small contig

Open jhcaddisfly opened this issue 5 years ago • 2 comments

Hallo,

I tried to reassemble a plastome but it gave me three contigs which are rather small. Do you have any ideas on how to improve the assembly? I took the plastome of a very closely related species as a reference. log_Adrian.txt config.txt

Best,

J.

jhcaddisfly avatar Dec 12 '19 12:12 jhcaddisfly

Hi, I would add a insert size, although doesn't have to be accurate, just put 300. I would also recommend to download the latest version 3.7.2 and to use a different seed, not just a complete chloroplast (try the RUBP gene seed I added on the github page)

ndierckx avatar Dec 12 '19 14:12 ndierckx

Thanks a lot!

jhcaddisfly avatar Dec 12 '19 15:12 jhcaddisfly