Use magicblast to map an experiment in the SRA reporitory

[sabina-llr]

Hi,

I have used magicblast version 1.4.0 and 1.5.0 in the past to map a query to experiments in the SRA repository. I have now installed the latestmagicblast version and ran a script (that gave me results a few month ago) to make sure that everything works as it is supposed to be. However, when running this:

makeblastdb -in reference.fa -out reference -parse_seqids -dbtype nucl magicblast -sra SRR8732225 -db reference -out SRR8732225_aligned.sam -num_threads 2 -outfmt sam -no_unaligned

I get the following error message: VDB: 2022-09-09T09:01:05 . sys: mbedtls_ssl_get_verify_result returned 0x4008 ( !! The certificate is not correctly signed by the trusted CA !! The certificate is signed with an unacceptable hash. ) BLAST query/options error: The provided SRA accession 'SRR8732225' does not exist Please refer to the BLAST+ user manual.

I have tried using several SRA experiments but I get the same error for all of them... any idea what the problem could be? Thanks for any helps!