Use NCBI SRA repository ( NCBI Magic-BLAST RNA-seq mapping tool)

[joseailtoncruz]

Hello how are you!

I'm trying to use the NCBI Magic-BLAST tool RNA-seq mapping tool, but I'm not getting it.

I followed the tutorial available on NCBI (https://ncbi.github.io/magicblast/cook/sra.html). Firstly I downloaded the package and installed it on my machine, then I created a database with my reference sequences (ie my refseq, several sequences, totaling 9,500).

magicblast -query SRR1237994.fas.gz -db my_reference

Soon after I tried to use the NCBI SRA repository, and the following error appeared: @PG ID:magicblast PN:magicblast CL: BLAST query/options error: FASTA parse error: defline expected Please refer to the BLAST+ user manual

I also tried to use it locally, downloading SRR..... and then running it via terminal with the following command: magicblast -query reads.fa -db my_reference. And again I get the same error.

I've tried several commands and so far I haven't been able to get any result. Could someone help me??

Thank you in advance, and I apologize for my English.