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-fr and -rf flags for strand specific RNA-seq

Open asethi-1 opened this issue 4 years ago • 3 comments

Hello,

I am mapping some paired-end strand-specific RNA-seq. This data maps logically with STAR, with the majority of reads in sense with their transcript. However, this is not the case in Magic-BLAST. Where can I find documentation on "-rf" and "-fr" flags for magic blast? How can they be used to achieve strand-specific mapping?

asethi-1 avatar Jun 14 '20 04:06 asethi-1