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"clean" function unmasks the genome?
Hello,
We have screened and cleaned many de novo assemblies with fcs-gx. We used the cleaning command:
cat ${FASTA} | python3 ${FCSGS_SCRIPT} --image ${IMAGE} --env-file /work/kwacker/env.txt \
clean genome \
--action-report ${REPORT} \
--output ${CLEAN_OUT} \
--contam-fasta-out ${CONTAM_OUT}
Repeats were soft-masked prior to screening, but appear to be completely unmasked in the output fasta. Is this supposed to happen? Is there a way to disable this behavior?
Thanks for your help.
The output of clean genome is uppercased fasta. Currently there's no way to disable it. We may add preserving of lowercase-softmasking in the future.