wgs2ncbi
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How to respond to validation issues
Hello, Working on using a genome for submission to NCBI contamination errors were sent back. Please how can some of the validation and contamination errors be solved using this tool?
These are the error groups:
- Genes completely overlapped by other genes
- FEATURE_LOCATION_CONFLICT: 6626 features have inconsistent gene locations.
- FEATURE_LOCATION_CONFLICT: RNA feature location does not match gene location
- FATAL: SHOW_HYPOTHETICAL_CDS_HAVING_GENE_NAME: 2 hypothetical coding regions have a gene name
- ERROR: valid [SEQ_INST.BadProteinStart] gap symbol at start of protein sequence
- ERROR: valid [SEQ_INST.StopInProtein] [27] termination symbols in protein sequence