Altered quality info so bowties can't map

[fgponce]

Hi, new to this biof stuff. But used sabre a few times on my fastqs and worked great (Thanks a Lot). However, last run....can use bowtie2 to map non demultiplexed fastq fine no errors. Demultiplex with sabre then about half the new fastqs throw errors saying "Error: Read M00733:9:000000000-A5T8E:1:2116:15800:22597 2:N:0:1 has more read characters than quality values. terminate called after throwing an instance of 'int' bowtie2-align died with signal 6 (ABRT) (core dumped)"

Got MiSeq to remake fastqs and tried again. Same problem. Deleted the entry for the error throwing line, found a new one. Gave up after deleting four reads worth.

Not sure what to do next!!!

Any help appreciated..

[lina2015]

You have to check the original fastaq file to see if there is something wrong during procesing your data,e.g. reads are not correctly cut in quality control. I met similar problem with you.

[maymystery]

I met similar problem with you too. Hope someone can help with it .